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Anti-CDH11 Antibody (6T438) is a Mouse antibody targeting CDH11. Anti-CDH11 Antibody (6T438) can be used in WB,ICC,IHC,FCM.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $296 | 7-10 days | |
100 μL | $428 | 7-10 days |
Description | Anti-CDH11 Antibody (6T438) is a Mouse antibody targeting CDH11. Anti-CDH11 Antibody (6T438) can be used in WB,ICC,IHC,FCM. |
Synonyms | Osteoblast cadherin, OSF-4, OSF4, OB-cadherin, OBcadherin, OB, CDHOB, Cdh11, Cadherin 11 type 2 OB cadherin (osteoblast), Cadherin 11 type 2, Cadherin 11, CAD11 |
Reactivity | Human |
Verified Activity | 1. Western blot analysis of CDH11 on human CDH11 recombinant protein using anti-CDH11 antibody at 1/1,000 dilution. 2. Western blot analysis of CDH11 on HEK293 (1) and CDH11-hIgGFc transfected HEK293 (2) cell lysate using anti-CDH11 antibody at 1/1,000 dilution. 3. Western blot analysis of CDH11 on MCF-7 (1), Jurkat (2) and HEK293 (3) cell lysate using anti-CDH11 antibody at 1/1,000 dilution. 4. Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue using anti-CDH11 antibody. Counter stained with hematoxylin. 5. ICC staining CDH11 (green) and Actin filaments (red) in HL-7702 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. Flow cytometric analysis of Hela cells with CDH11 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red). |
Application | |
Recommended Dose | WB: 1:500-2000; IHC: 1:50-200; ICC: 1:50-200; FCM: 1:50-100 |
Antibody Type | Monoclonal |
Host Species | Mouse |
Construction | Hybridoma Monoclonal Antibody |
Purification | ProA affinity purified |
Appearance | Liquid |
Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
Research Background | Cadherins are calcium dependent cell adhesion proteins, a family of Ca++-dependent adhesion molecules that influence cell-cell binding and are critical to the maintenance of tissue structure and morphogenesis. OB-cadherin (osteoblast-cadherin, cadherin-11, OSF-4) has two forms, OB-cadherin-1 and OB-cadherin-2. OB-cadherin-2 has a truncated cytoplasmic domain, missing amino acids 694-796. Both OB-cadherins are expressed in osteoblastic cell lines with low expression seen in lungs, testis and brain. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types |
Conjucates | Unconjugated |
Immunogen | Recombinant Protein |
Uniprot ID |
Molecular Weight | Theoretical: 88 kDa. |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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