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Anti-CDCP1 Antibody (9F26) is a Mouse antibody targeting CDCP1. Anti-CDCP1 Antibody (9F26) can be used in WB.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $298 | 7-10 days | |
100 μL | $426 | 7-10 days |
Description | Anti-CDCP1 Antibody (9F26) is a Mouse antibody targeting CDCP1. Anti-CDCP1 Antibody (9F26) can be used in WB. |
Synonyms | TRASK, SIMA135, CUB domain containing protein 1, CD318 |
Reactivity | Human,,Mouse |
Verified Activity | 1. Western blot analysis of CDCP1 on different lysates at 1/1,000 dilution. Lane 1: SW480 cell, lysate Lane 2: HCT 116 cell lysate, Lane 3: NIH/3T3 cell lysate (no heat) Lysates/proteins at 20 μg/Lane. Predicted band size: 93 kDa. Observed band size: 130 kDa. Exposure time: Lane 1-2: 24 seconds; Lane 3: 10 seconds; 4-20% SDS-PAGE gel. 2. Western blot analysis of CDCP1 on different lysates at 1/1,000 dilution. Lane 1: HCT 116 cell lysate, Lane 2: HCT 116 cell lysate treated with deglycosylation Lysates/proteins at 14 μg/Lane. Predicted band size: 93 kDa. Observed band size: 93/130 kDa (Glycosylated). Exposure time: 3 minutes; 4-20% SDS-PAGE gel. |
Application | |
Recommended Dose | WB: 1:1000 |
Antibody Type | Monoclonal |
Host Species | Mouse |
Construction | Hybridoma Monoclonal Antibody |
Purification | Immunogen affinity purified |
Appearance | Liquid |
Formulation | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Research Background | CDCP1 (CUB-domain-containing protein 1) contains three extracellular CUB domains, a transmembrane domain, and two putative cytoplasmic tyrosine phosphorylation sites. Phosphorylation of the gp140 and p80 proteins is mediated by Src family kinases at various tyrosine residues, including Tyr 734. PTP family members mediate the desphosphorylation of CDCP1. The conversion of gp140 to p80 prolongs the phosphorylation state, which may affect signaling in epithelial wounds. CDCP1 acts as a marker for hematopoetic cells and also exhibits high expression in metastatic colon and breast tumors. |
Conjucates | Unconjugated |
Immunogen | Recombinant Protein: Human CDCP1 |
Antigen Species | human |
Uniprot ID |
Molecular Weight | Theoretical: 93 kDa. |
Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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