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Anti-Cdc7 Polyclonal Antibody
Catalog No. TMAB-04138 Copy Product Info
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Anti-Cdc7 Polyclonal Antibody is a Rabbit antibody targeting Cdc7. Anti-Cdc7 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF,FCM.
Anti-Cdc7 Polyclonal Antibody
Copy Product Info🥰Excellent
Catalog No. TMAB-04138
Anti-Cdc7 Polyclonal Antibody is a Rabbit antibody targeting Cdc7. Anti-Cdc7 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF,FCM.
Anti-Cdc7 Polyclonal Antibody
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 50 μL | $222 | 7-10 days | 7-10 days | |
| 100 μL | $373 | 7-10 days | 7-10 days | |
| 200 μL | $527 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
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| Description | Anti-Cdc7 Polyclonal Antibody is a Rabbit antibody targeting Cdc7. Anti-Cdc7 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF,FCM. |
| Ig Type | IgG |
| Reactivity | Human (predicted:Mouse,Rat,Dog,Pig,Cow,Horse) |
| Verified Activity | 1. Tissue/cell: human glioma tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min; Incubation: Anti-Cdc7 Polyclonal Antibody, Unconjugated (TMAB-04138) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining 2. Blank control (blue): 293T (fixed with 2% paraformaldehyde (10 min)). Primary Antibody: Rabbit Anti-Cdc7/FITC Conjugated antibody (TMAB-04138 /FITC), Dilution: 1 μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/FITC (orange), used under the same conditions. |
| Application | |
| Recommended Dose | IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1μg/Test |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Nucleus. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | DNA replication in eukaryotic cells is dependent on the phosphorylation of the pre-replicative complex (preRC) at the origin of replication. Two complexes of proteins mediate this event, the cyclin dependent kinase (CDK) complex, and the Cdc7 kinase-ASK complex. The activity of Cdc7 kinase oscillates during cell cycle. The major targets of Cdc7 kinase are proteins that belong to the MCM complex (mini chromosome maintenance proteins). Cdc7 kinase was also found to be important in meiosis, checkpoint responses, maintenance of chromosome structure, and repair. |
| Immunogen | KLH conjugated synthetic peptide: human Cdc7 |
| Antigen Species | Human |
| Gene Name | CDC7 |
| Gene ID | |
| Protein Name | Cell division cycle 7-related protein kinase |
| Uniprot ID | |
| Function | Seems to phosphorylate critical substrates that regulatethe G1/S phase transition and/or DNA replication. Canphosphorylates MCM2 and MCM3. |
| Molecular Weight | Theoretical: 64 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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