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Anti-CDC45L Polyclonal Antibody
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Catalog No. TMAB-04132
Anti-CDC45L Polyclonal Antibody is a Rabbit antibody targeting CDC45L. Anti-CDC45L Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
Anti-CDC45L Polyclonal Antibody
Anti-CDC45L Polyclonal Antibody
Copy Product Info😃Good
Catalog No. TMAB-04132
Anti-CDC45L Polyclonal Antibody is a Rabbit antibody targeting CDC45L. Anti-CDC45L Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $221 | 7-10 days | 7-10 days | |
| 100 μL | $374 | 7-10 days | 7-10 days | |
| 200 μL | $528 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Antigen Details
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| Description | Anti-CDC45L Polyclonal Antibody is a Rabbit antibody targeting CDC45L. Anti-CDC45L Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Mouse,Rat (predicted:Human,Pig,Horse) |
| Verified Activity | 1. Tissue/cell: Rat brain; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min; Incubation: Anti-CDC45L Polyclonal Antibody, Unconjugated (TMAB-04132) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining 2. Tissue/cell: mouse embryo tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min; Incubation: Anti-CDC45L Polyclonal Antibody, Unconjugated (TMAB-04132) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining 3. Paraformaldehyde-fixed, paraffin embedded (mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CDC45L) Polyclonal Antibody, Unconjugated (TMAB-04132) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. |
| Application | |
| Recommended Dose | IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasm. Nucleus. |
| Tissue Specificity | Widely expressed, highest levels are found in adult testis and thymus and in fetal liver. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | The protein encoded by this gene was identified by its strong similarity with Saccharomyces cerevisiae Cdc45, an essential protein required to the initiation of DNA replication. Cdc45 is a member of the highly conserved multiprotein complex including Cdc6/Cdc18, the minichromosome maintenance proteins (MCMs) and DNA polymerase, which is important for early steps of DNA replication in eukaryotes. This protein has been shown to interact with MCM7 and DNA polymerase alpha. Studies of the similar gene in Xenopus suggested that this protein play a pivotal role in the loading of DNA polymerase alpha onto chromatin. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
| Immunogen | KLH conjugated synthetic peptide: human CDC45L |
| Antigen Species | Human |
| Gene Name | CDC45 |
| Gene ID | |
| Protein Name | Cell division control protein 45 homolog |
| Uniprot ID | |
| Function | Required for initiation of chromosomal DNA replication. |
| Molecular Weight | Theoretical: 66 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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