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Anti-CDC42 Antibody (2L809) is a Rabbit antibody targeting CDC42. Anti-CDC42 Antibody (2L809) can be used in WB,IHC,IP,FCM.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $296 | 7-10 days | |
100 μL | $497 | 7-10 days |
Description | Anti-CDC42 Antibody (2L809) is a Rabbit antibody targeting CDC42. Anti-CDC42 Antibody (2L809) can be used in WB,IHC,IP,FCM. |
Synonyms | G25K, cell division cycle 42, CDC42Hs |
Ig Type | IgG |
Reactivity | Human,Mouse,Rat |
Verified Activity | 1. Western blot analysis of CDC42 on different lysates using anti-CDC42 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela, Lane 2: Jurkat, Lane 3: HepG2. 2. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-CDC42 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-CDC42 antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-CDC42 antibody. Counter stained with hematoxylin. 5. Flow cytometric analysis of Hela cells with CDC42 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
Application | |
Recommended Dose | WB: 1:1000; IHC: 1:50-200; FCM: 1:50-100 |
Antibody Type | Monoclonal |
Host Species | Rabbit |
Construction | Recombinant Antibody |
Purification | ProA affinity purified |
Appearance | Liquid |
Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
Research Background | The superfamily of GTP-binding proteins, for which the Ras proteins are prototypes, has been implicated in regulation of diverse biological activities involving various aspects of cell growth and division. One mammalian member of the family, Cdc42, has an amino acid sequence that is similar to those of various members of the Ras superfamily proteins, including N-, K- and H-Ras, Rho proteins and the Rac proteins. On the basis of in vitro phosphorylation studies, it has been suggested that human Cdc42 may function in the signaling pathway of the EGF receptor or related growth factor receptor protein kinases. The Dbl oncogene has been shown to specifically catalyze dissociation of GDP from human Cdc42. |
Conjucates | Unconjugated |
Immunogen | Recombinant Protein |
Uniprot ID |
Molecular Weight | Theoretical: 21 kDa. |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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