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Anti-CDC34 Antibody (7P182)
😃Good
Catalog No. TMAC-00767
Alias Ubiquitin-protein ligase R1, Ubiquitin-conjugating enzyme E2-CDC34, Ubiquitin-conjugating enzyme E2-32 kDa complementing, Ubiquitin-conjugating enzyme E2 R1, Ubiquitin protein ligase R1, Ubiquitin protein ligase, Ubiquitin conjugating enzyme E2 32 kDa complementing, Ubiquitin conjugating enzyme Cdc34, Ubiquitin carrier protein, UBE2R1, UBE2 R1, UBCH3, UBC3, UBC 3, UB2R1_HUMAN, E2 CDC34, Cell division cycle 34 homolog (S. cerevisiae), Cell division cycle 34 homolog, Cell division cycle 34, Cdc34, Cdc 34
Anti-CDC34 Antibody (7P182) is a Rabbit antibody targeting CDC34. Anti-CDC34 Antibody (7P182) can be used in WB,ICC/IF,IP,FCM.
Anti-CDC34 Antibody (7P182)
Anti-CDC34 Antibody (7P182)
😃Good
Catalog No. TMAC-00767Alias Ubiquitin-protein ligase R1, Ubiquitin-conjugating enzyme E2-CDC34, Ubiquitin-conjugating enzyme E2-32 kDa complementing, Ubiquitin-conjugating enzyme E2 R1, Ubiquitin protein ligase R1, Ubiquitin protein ligase, Ubiquitin conjugating enzyme E2 32 kDa complementing, Ubiquitin conjugating enzyme Cdc34, Ubiquitin carrier protein, UBE2R1, UBE2 R1, UBCH3, UBC3, UBC 3, UB2R1_HUMAN, E2 CDC34, Cell division cycle 34 homolog (S. cerevisiae), Cell division cycle 34 homolog, Cell division cycle 34, Cdc34, Cdc 34
Anti-CDC34 Antibody (7P182) is a Rabbit antibody targeting CDC34. Anti-CDC34 Antibody (7P182) can be used in WB,ICC/IF,IP,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $297 | 7-10 days | |
| 100 μL | $497 | 7-10 days |
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Product Introduction
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Antigen Details
Chemical Properties
| Description | Anti-CDC34 Antibody (7P182) is a Rabbit antibody targeting CDC34. Anti-CDC34 Antibody (7P182) can be used in WB,ICC/IF,IP,FCM. |
| Synonyms | Ubiquitin-protein ligase R1, Ubiquitin-conjugating enzyme E2-CDC34, Ubiquitin-conjugating enzyme E2-32 kDa complementing, Ubiquitin-conjugating enzyme E2 R1, Ubiquitin protein ligase R1, Ubiquitin protein ligase, Ubiquitin conjugating enzyme E2 32 kDa complementing, Ubiquitin conjugating enzyme Cdc34, Ubiquitin carrier protein, UBE2R1, UBE2 R1, UBCH3, UBC3, UBC 3, UB2R1_HUMAN, E2 CDC34, Cell division cycle 34 homolog (S. cerevisiae), Cell division cycle 34 homolog, Cell division cycle 34, Cdc34, Cdc 34 |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Western blot analysis of Cdc34 on different lysates using anti-Cdc34 antibody at 1/1,000 dilution. Positive control: Lane 1: K562, Lane 2: Jurkat. 2. ICC staining Cdc34 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 3. ICC staining Cdc34 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 4. ICC staining Cdc34 in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 5. Flow cytometric analysis of Jurkat cells with Cdc34 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
| Application | |
| Recommended Dose | WB: 1:1000-5000; ICC/IF: 1:100-500; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | Cell cycle events are regulated by the sequential activation and deactivation of cyclin dependent kinases (Cdks) and by the proteolysis of cyclins. The cell division cycle (Cdc) genes are required at various points in the cell cycle. Cdc25A, Cdc25B and Cdc25C protein tyrosine phosphatases function as mitotic activators by dephosphorylating Cdc2 p34 on regulatory tyrosine residues. Cdc6 is the human homolog of Saccharomyces cerevisiae Cdc6, which is involved in the initiation of DNA replication. Cdc37 appears to facilitate Cdk4/cyclin D1 complex formation and has been shown to form a stable complex with Hsp90. Cdc34, Cdc27 and Cdc16 function as ubiquitin-conjugating enzymes. Cdc34 is thought to be the structural and functional homolog of Saccharomyces cerevisiae Cdc34, which is essential for the G1 to S phase transition. Cdc16 and Cdc27 are components of the APC (anaphase-promoting complex) which ubiquitinates cyclin B, resulting in cyclin B/Cdk complex degradation. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 34 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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