Anti-Cdc25C Antibody (9N768) is a Rabbit antibody targeting Cdc25C. Anti-Cdc25C Antibody (9N768) can be used in WB,ICC/IF,IP.

IgG

9N768

Human

1. 25 μg total protein per lane of various lysates (see on figure) probed with Cdc25C monoclonal antibody, unconjugated (TMAB-04121) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
2. 4% Paraformaldehyde-fixed K562 (H) cell; Triton X-100 at r. T. for 20 min; Antibody incubation with (CDC25C) monoclonal Antibody, unconjugated (TMAB-04121) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

WB: 1:500-2000; ICC/IF: 1:50-200; IP: 1:20-50

Monoclonal

A synthesized peptide: human CDC25C

Human

CDC25C

M-phase inducer phosphatase 3

Functions as a dosage-dependent inducer in mitotic control. Tyrosine protein phosphatase required for progression of the cell cycle. When phosphorylated, highly effective in activating G2 cells into prophase. Directly dephosphorylates CDK1 and activates its kinase activity.