Anti-Cdc25B Polyclonal Antibody is a Rabbit antibody targeting Cdc25B. Anti-Cdc25B Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Mouse,Rat (predicted:Dog,Pig,Cow,Rabbit)

1. Sample: Spleen (Mouse) Lysate at 40 μg
Primary: Anti-Cdc25B (TMAB-04117) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 65 kD
Observed band size: 65 kD
2. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Cdc25B ) Polyclonal Antibody, Unconjugated (TMAB-04117) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
3. Sample:
Lane 1: Mouse Liver tissue lysates
Lane 2: Mouse NIH/3T3 cell lysates
Lane 3: Mouse Sp2/0 cell lysates
Lane 4: Human A549 cell lysates
Lane 5: Human Daudi cell lysates
Lane 6: Human K562 cell lysates
Primary: Anti-Cdc25B (TMAB-04117) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 65 kDa
Observed band size: 60 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human Cdc25B

Human

CDC25B

M-phase inducer phosphatase 2

Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Directly dephosphorylates CDK1 and stimulates its kinase activity. The three isoforms seem to have a different level of activity.