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Anti-Cdc25B Polyclonal Antibody

🥰Excellent
Catalog No. TMAB-04117

Antibody Type: Rabbit Polyclonal
Application: WB,IHC-P,IHC-Fr,IF
Reactivity: Human,Mouse,Rat (predicted:Dog,Pig,Cow,Rabbit)

Anti-Cdc25B Polyclonal Antibody

Anti-Cdc25B Polyclonal Antibody

🥰Excellent
Catalog No. TMAB-04117
Antibody Type: Rabbit Polyclonal
Application: WB,IHC-P,IHC-Fr,IF
Reactivity: Human,Mouse,Rat (predicted:Dog,Pig,Cow,Rabbit)
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Product Introduction

Bioactivity
Description
Anti-Cdc25B Polyclonal Antibody is a Rabbit antibody targeting Cdc25B. Anti-Cdc25B Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
Reactivity
Human,Mouse,Rat (predicted:Dog,Pig,Cow,Rabbit)
Application
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationCytoplasm, cytoskeleton, centrosome. Cytoplasm, cytoskeleton, spindle pole.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundCDC25B is a member of the CDC25 family of phosphatases. CDC25B activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. CDC25B shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined. Multiple transcript variants for this gene exist. [provided by RefSeq, Jul 2008].
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human Cdc25B
Antigen Species
Human
Gene Name
CDC25B
Gene ID
Protein Name
M-phase inducer phosphatase 2
Uniprot ID
Function
Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Directly dephosphorylates CDK1 and stimulates its kinase activity. The three isoforms seem to have a different level of activity.
Chemical Properties
Molecular WeightTheoretical: 65 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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