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Anti-Cdc25B Polyclonal Antibody
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Catalog No. TMAB-04117 Copy Product Info
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Anti-Cdc25B Polyclonal Antibody is a Rabbit antibody targeting Cdc25B. Anti-Cdc25B Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-Cdc25B Polyclonal Antibody
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 50 µL | $221 | 7-10 days | 7-10 days | |
| 100 µL | $371 | 7-10 days | 7-10 days | |
| 200 µL | $529 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
Stability & Storage
| Description | Anti-Cdc25B Polyclonal Antibody is a Rabbit antibody targeting Cdc25B. Anti-Cdc25B Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat (predicted:Dog,Pig,Cow,Rabbit) |
| Verified Activity | 1. Sample: Spleen (Mouse) Lysate at 40 μg Primary: Anti-Cdc25B (TMAB-04117) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 65 kD Observed band size: 65 kD 2. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Cdc25B ) Polyclonal Antibody, Unconjugated (TMAB-04117) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody for 20 minutes and DAB staining. 3. Sample: Lane 1: Mouse Liver tissue lysates Lane 2: Mouse NIH/3T3 cell lysates Lane 3: Mouse Sp2/0 cell lysates Lane 4: Human A549 cell lysates Lane 5: Human Daudi cell lysates Lane 6: Human K562 cell lysates Primary: Anti-Cdc25B (TMAB-04117) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 65 kDa Observed band size: 60 kDa |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasm, cytoskeleton, centrosome. Cytoplasm, cytoskeleton, spindle pole. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | CDC25B is a member of the CDC25 family of phosphatases. CDC25B activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. CDC25B shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined. Multiple transcript variants for this gene exist. [provided by RefSeq, Jul 2008]. |
| Immunogen | KLH conjugated synthetic peptide: human Cdc25B |
| Antigen Species | Human |
| Gene Name | CDC25B |
| Gene ID | |
| Protein Name | M-phase inducer phosphatase 2 |
| Uniprot ID | |
| Function | Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Directly dephosphorylates CDK1 and stimulates its kinase activity. The three isoforms seem to have a different level of activity. |
| Molecular Weight | Theoretical: 65 kDa. Actual: 60 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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