Anti-CD97 Antibody (2F447) is a Rabbit antibody targeting CD97. Anti-CD97 Antibody (2F447) can be used in FCM, ICC/IF, IP, WB.

IgG

2F447

Human (predicted:Mouse)

1. 4% Paraformaldehyde-fixed Jurkat (H) cell; Antibody incubation with (CD97) monoclonal Antibody, unconjugated (TMAB-04104) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
2. The Jurkat (H) cells were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.). Primary Antibody (green): Rabbit Anti-CD97 antibody (TMAB-04104, 1:100); Secondary Antibody (white blue): Goat anti-Rabbit IgG-BF488: 1 μg/test. Blank control (black): PBS. Acquisition of 20,000 events was performed.

FCM=1:50-100; ICC/IF=1:50-200; IP=1:20-50; WB=1:500-2000

Monoclonal

A synthesized peptide: human ADGRE5

Human

ADGRE5

Adhesion G protein-coupled receptor E5

Receptor potentially involved in both adhesion and signaling processes early after leukocyte activation. Plays an essential role in leukocyte migration.