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Anti-CD90/Thy-1 Antibody (6F192)

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Catalog No. TMAB-04100

Anti-CD90/Thy-1 Antibody (6F192) is a Rabbit antibody targeting CD90/Thy-1. Anti-CD90/Thy-1 Antibody (6F192) can be used in WB,IHC-P,IHC-Fr,IF.

Anti-CD90/Thy-1 Antibody (6F192)

Anti-CD90/Thy-1 Antibody (6F192)

Copy Product Info
😃Good
Catalog No. TMAB-04100
Anti-CD90/Thy-1 Antibody (6F192) is a Rabbit antibody targeting CD90/Thy-1. Anti-CD90/Thy-1 Antibody (6F192) can be used in WB,IHC-P,IHC-Fr,IF.
Pack SizePriceUSA WarehouseGlobal WarehouseQuantity
25 μL$1487-10 days7-10 days
50 μL$2637-10 days7-10 days
100 μL$4717-10 days7-10 days
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction

Bioactivity
Description
Anti-CD90/Thy-1 Antibody (6F192) is a Rabbit antibody targeting CD90/Thy-1. Anti-CD90/Thy-1 Antibody (6F192) can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
Clone
6F192
Reactivity
Rat,Mouse,Human
Verified Activity
1. Sample:
Lane 1: Mouse Cerebrum tissue lysates
Lane 2: Rat Thymus tissue lysates
Primary: Anti-CD90/Thy-1 (TMAB-04100) at 1/2000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 12 kDa
Observed band size: 25 kDa
2. Paraformaldehyde-fixed, paraffin embedded Human Glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD90/Thy1 Monoclonal Antibody, Unconjugated (TMAB-04100) at 1:100 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD90/Thy1 Monoclonal Antibody, Unconjugated (TMAB-04100) at 1:100 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD90/Thy1 Monoclonal Antibody, Unconjugated (TMAB-04100) at 1:100 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD90/Thy1 Monoclonal Antibody, Unconjugated (TMAB-04100) at 1:100 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD90/Thy-1 Monoclonal Antibody, Unconjugated (TMAB-04100) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
7. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD90/Thy-1 Monoclonal Antibody, Unconjugated (TMAB-04100) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
Application
Recommended Dose
WB: 1:1000-5000; IHC-P: 1:50-200; IHC-Fr: 1:50-200; IF: 1:50-200
Antibody Type
Monoclonal
Host SpeciesRabbit
Subcellular LocalizationCell membrane; Lipid-anchor, GPI-anchor.
Tissue SpecificityAbundant in lymphoid tissues.
ConstructionRecombinant Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundThy-1 or CD90 (Cluster of Differentiation 90) is a 25–37 kDa heavily N-glycosylated, glycophosphatidylinositol (GPI) anchored conserved cell surface protein with a single V-like immunoglobulin domain, originally discovered as a thymocyte antigen. Thy-1can be used as a marker for a variety of stem cells and for the axonal processes of mature neurons. Structural study of Thy-1 lead to the foundation of the Immunoglobulin superfamily, of which it is the smallest member, and led to some of the initial biochemical description and characterization of a vertebrate GPI anchor and also the first demonstration of tissue specific differential glycosylation.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human CD90/Thy-1
Antigen Species
Human
Gene Name
THY1
Gene ID
Protein Name
Thy-1 membrane glycoprotein
Uniprot ID
Function
May play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain.
Chemical Properties
Molecular WeightTheoretical: 12 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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