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Anti-CD9 Polyclonal Antibody is a Rabbit antibody targeting CD9. Anti-CD9 Polyclonal Antibody can be used in WB,FCM.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $220 | 7-10 days | |
100 μL | $372 | 7-10 days | |
200 μL | $529 | 7-10 days |
Description | Anti-CD9 Polyclonal Antibody is a Rabbit antibody targeting CD9. Anti-CD9 Polyclonal Antibody can be used in WB,FCM. |
Ig Type | IgG |
Reactivity | Human,Mouse,Rat (predicted:Dog,Pig,Cow,Rabbit,Sheep) |
Verified Activity | 1. Blank control: Raji (blue). Primary Antibody: Rabbit Anti-CD9 antibody (TMAB-00397), Dilution: 5 μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG (orange),used under the same conditions. Secondary Antibody: Goat anti-rabbit IgG-Pe (white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. 2. Blank control: RSC96 cells (blue). Primary Antibody: Rabbit Anti-CD9 antibody (TMAB-00397), Dilution: 1 μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit Igg (orange),used under the same conditions); Secondary Antibody: Goat anti-rabbit IgG-Pe (white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol Primary antibody were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 10% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. 3. Blank control: Mouse spleen cells (blue). Primary Antibody: Rabbit Anti-CD9 antibody (TMAB-00397), Dilution: 1 μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit Igg (orange),used under the same conditions); Secondary Antibody: Goat anti-rabbit IgG-Pe (white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol Primary antibody were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 10% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. 4. Sample: Lane 1: Testis (Mouse) Lysate at 40 μg Lane 2: Placenta (Mouse) Lysate at 40 μg Lane 3: Testis (Rat) Lysate at 40 μg Primary: Anti-CD9 (TMAB-00397) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 24 kDa Observed band size: 24 kDa |
Application | WB,FCM |
Recommended Dose | WB: 1:500-2000; FCM: 1μg/Test |
Antibody Type | Polyclonal |
Host Species | Rabbit |
Subcellular Localization | Membrane; Multi-pass membrane protein. |
Construction | Hybridoma Polyclonal Antibody |
Purification | Protein A purified |
Appearance | Liquid |
Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
Research Background | This gene encodes a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Tetraspanins are cell surface glycoproteins with four transmembrane domains that form multimeric complexes with other cell surface proteins. The encoded protein functions in many cellular processes including differentiation, adhesion, and signal transduction, and expression of this gene plays a critical role in the suppression of cancer cell motility and metastasis. [provided by RefSeq, Jan 2011] |
Immunogen | KLH conjugated synthetic peptide: human CD9 |
Antigen Species | Human |
Gene ID | |
Uniprot ID | |
Biology Area | Blood,Platelets,Intracellular,Fertilisation,Platelets,Surface Molecules |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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