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Anti-CD9 Polyclonal Antibody

😃Good
Catalog No. TMAB-00397

Anti-CD9 Polyclonal Antibody is a Rabbit antibody targeting CD9. Anti-CD9 Polyclonal Antibody can be used in WB,FCM.

Anti-CD9 Polyclonal Antibody

Anti-CD9 Polyclonal Antibody

😃Good
Catalog No. TMAB-00397
Anti-CD9 Polyclonal Antibody is a Rabbit antibody targeting CD9. Anti-CD9 Polyclonal Antibody can be used in WB,FCM.
Pack SizePriceAvailabilityQuantity
50 μL$220 7-10 days
100 μL$372 7-10 days
200 μL$529 7-10 days
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Product Introduction

Bioactivity
Description
Anti-CD9 Polyclonal Antibody is a Rabbit antibody targeting CD9. Anti-CD9 Polyclonal Antibody can be used in WB,FCM.
Ig Type
IgG
Reactivity
Human,Mouse,Rat (predicted:Dog,Pig,Cow,Rabbit,Sheep)
Verified Activity
1. Blank control: Raji (blue).
Primary Antibody: Rabbit Anti-CD9 antibody (TMAB-00397), Dilution: 5 μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG (orange),used under the same conditions.
Secondary Antibody: Goat anti-rabbit IgG-Pe (white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
2. Blank control: RSC96 cells (blue). Primary Antibody: Rabbit Anti-CD9 antibody (TMAB-00397), Dilution: 1 μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit Igg (orange),used under the same conditions); Secondary Antibody: Goat anti-rabbit IgG-Pe (white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Protocol
Primary antibody were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 10% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice.
3. Blank control: Mouse spleen cells (blue). Primary Antibody: Rabbit Anti-CD9 antibody (TMAB-00397), Dilution: 1 μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit Igg (orange),used under the same conditions); Secondary Antibody: Goat anti-rabbit IgG-Pe (white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Protocol
Primary antibody were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 10% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice.
4. Sample:
Lane 1: Testis (Mouse) Lysate at 40 μg
Lane 2: Placenta (Mouse) Lysate at 40 μg
Lane 3: Testis (Rat) Lysate at 40 μg
Primary:
Anti-CD9 (TMAB-00397) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 24 kDa
Observed band size: 24 kDa
Application
WB,FCM
Recommended Dose
WB: 1:500-2000; FCM: 1μg/Test
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationMembrane; Multi-pass membrane protein.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundThis gene encodes a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Tetraspanins are cell surface glycoproteins with four transmembrane domains that form multimeric complexes with other cell surface proteins. The encoded protein functions in many cellular processes including differentiation, adhesion, and signal transduction, and expression of this gene plays a critical role in the suppression of cancer cell motility and metastasis. [provided by RefSeq, Jan 2011]
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human CD9
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Blood,Platelets,Intracellular,Fertilisation,Platelets,Surface Molecules
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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