Anti-CD86 Polyclonal Antibody 2 is a Rabbit antibody targeting CD86. Anti-CD86 Polyclonal Antibody 2 can be used in WB,FCM,ICC/IF.

IgG

Human,Mouse,Rat

1. 25 μg total protein per lane of various lysates (see on figure) probed with CD86 polyclonal antibody, unconjugated (TMAB-04087) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
2. 4% Paraformaldehyde-fixed Daudi (H) cell; Antibody incubation with (CD86) polyclonal Antibody, unconjugated (TMAB-04087) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
3. The MJ (H) cells were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-CD86 antibody (TMAB-04087): 1 μg/10^6 cells; Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.

WB: 1:500-2000; FCM: 1μg/Test; ICC/IF: 1:50-200

Polyclonal

Recombinant Protein: human CD86 protein

Human

CD86

T-lymphocyte activation antigen CD86

Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation.