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Anti-CD80 Polyclonal Antibody 3 (Synonyms: LAB7, CD80 molecule, CD80, CD28LG1, BB1, B7-1, B71, B7.1, B7)
Catalog No. TMAB-00394 Copy Product Info
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Anti-CD80 Polyclonal Antibody 3 is a Rabbit antibody targeting CD80. Anti-CD80 Polyclonal Antibody 3 can be used in IF,IHC-Fr,IHC-P,WB.
Anti-CD80 Polyclonal Antibody 3
Copy Product Info🥰Excellent
Catalog No. TMAB-00394
Synonyms LAB7, CD80 molecule, CD80, CD28LG1, BB1, B7-1, B71, B7.1, B7
Anti-CD80 Polyclonal Antibody 3 is a Rabbit antibody targeting CD80. Anti-CD80 Polyclonal Antibody 3 can be used in IF,IHC-Fr,IHC-P,WB.
Anti-CD80 Polyclonal Antibody 3
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 50 μL | $222 | 7-10 days | 7-10 days | |
| 100 μL | $374 | 7-10 days | 7-10 days | |
| 200 μL | $527 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
Stability & Storage
| Description | Anti-CD80 Polyclonal Antibody 3 is a Rabbit antibody targeting CD80. Anti-CD80 Polyclonal Antibody 3 can be used in IF,IHC-Fr,IHC-P,WB. |
| Synonyms | LAB7, CD80 molecule, CD80, CD28LG1, BB1, B7-1, B71, B7.1, B7 |
| Ig Type | IgG |
| Reactivity | Human |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (Human melanoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CD80) Polyclonal Antibody, Unconjugated (TMAB-00394) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 2. Sample: U937 (Human) Cell Lysate at 30 μg Primary: Anti-CD80 (TMAB-00394) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 32 kDa Observed band size: 32 kDa 3. Sample: Raji (Human) Cell Lysate at 30 μg Primary: Anti-CD80 (TMAB-00394) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 32 kDa Observed band size: 32 kDa    |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Membrane; Single-pass type I membrane protein. |
| Tissue Specificity | Expressed on activated B-cells, macrophages and dendritic cells. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1 mg/mL |
| Research Background | CD80 is a member of the Ig superfamily and serves as the ligand for two T cell molecules, CD28 and CTLA4. Interactions between CD28 and CD80 on activated B cells result in enhanced T cell activation. CD80 is rapidly induced on the surface of in vitro activated B cells, Epstein Barr Virus (EBV) transformed B cell lines, Burkitts lymphoma cell lines, freshly isolated follicular B lymphoma cells, T cells, and monocytes. It is also expressed at high levels in dendritic cells. It reacts weakly with a small proportion of non activated normal B cells and with HTLV1 infected T cells. CD80 does not react with peripheral monocytes, resting and activated normal T cells, T cell lines and T cell clones, nor with myelomonocytic cell lines. |
| Immunogen | KLH conjugated synthetic peptide: human CD80 |
| Antigen Species | Human |
| Gene Name | CD80 |
| Gene ID | |
| Protein Name | T-lymphocyte activation antigen CD80 |
| Uniprot ID | |
| Biology Area | CD,B Lymphocytic Lineage,Dendritic Cell Lineage,Monocytic Lineage |
| Function | Involved in the costimulatory signal essential for T lymphocytes activation. T-cell proliferation and cytokine production is induced by the binding of CD28 or CTLA-4 to this receptor. |
| Molecular Weight | Theoretical: 32 kDa. |
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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