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Anti-CD80 Polyclonal Antibody 3

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Catalog No. TMAB-00394

Anti-CD80 Polyclonal Antibody 3 is a Rabbit antibody targeting CD80. Anti-CD80 Polyclonal Antibody 3 can be used in WB,IHC-P,IHC-Fr,IF.

Anti-CD80 Polyclonal Antibody 3

Anti-CD80 Polyclonal Antibody 3

😃Good
Catalog No. TMAB-00394
Anti-CD80 Polyclonal Antibody 3 is a Rabbit antibody targeting CD80. Anti-CD80 Polyclonal Antibody 3 can be used in WB,IHC-P,IHC-Fr,IF.
Pack SizePriceAvailabilityQuantity
50 μL$222 7-10 days
100 μL$374 7-10 days
200 μL$527 7-10 days
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Product Introduction

Bioactivity
Description
Antibody Type: Rabbit Polyclonal

Application: WB,IHC-P,IHC-Fr,IF

Reactivity: Human
Ig Type
IgG
Reactivity
Human
Verified Activity
1. Paraformaldehyde-fixed, paraffin embedded (Human melanoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CD80) Polyclonal Antibody, Unconjugated (TMAB-00394) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
2. Sample:
U937 (Human) Cell Lysate at 30 μg
Primary: Anti-CD80 (TMAB-00394) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 32 kDa
Observed band size: 32 kDa
3. Sample:
Raji (Human) Cell Lysate at 30 μg
Primary: Anti-CD80 (TMAB-00394) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 32 kDa
Observed band size: 32 kDa
Application
WB,IHC-P,IHC-Fr,IF
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationMembrane; Single-pass type I membrane protein.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human CD80
Antigen Species
Human
Gene ID
941
Uniprot ID
Biology Area
CD,B Lymphocytic Lineage,Dendritic Cell Lineage,Monocytic Lineage
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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