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Anti-CD8 alpha Antibody (9O552)
😃Good
Catalog No. TMAB-00391
Alias p32, OKT8 T-cell antigen, MAL, Lyt-2, Leu2, CD8a molecule, CD8a, CD8 α/CD8A, CD8
Anti-CD8 alpha Antibody (9O552) is a Rabbit antibody targeting CD8 alpha. Anti-CD8 alpha Antibody (9O552) can be used in WB,IHC-P,IHC-Fr,IF.
Anti-CD8 alpha Antibody (9O552)
Anti-CD8 alpha Antibody (9O552)
😃Good
Catalog No. TMAB-00391Alias p32, OKT8 T-cell antigen, MAL, Lyt-2, Leu2, CD8a molecule, CD8a, CD8 α/CD8A, CD8
Anti-CD8 alpha Antibody (9O552) is a Rabbit antibody targeting CD8 alpha. Anti-CD8 alpha Antibody (9O552) can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 25 μL | $154 | 7-10 days | |
| 50 μL | $273 | 7-10 days | |
| 100 μL | $489 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-CD8 alpha Antibody (9O552) is a Rabbit antibody targeting CD8 alpha. Anti-CD8 alpha Antibody (9O552) can be used in WB,IHC-P,IHC-Fr,IF. |
| Alias | p32, OKT8 T-cell antigen, MAL, Lyt-2, Leu2, CD8a molecule, CD8a, CD8 α/CD8A, CD8 |
| Ig Type | IgG |
| Clone | 9O552 |
| Reactivity | Human |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (human tonsil); Antigen retrieval by boiling in EDTA buffer (pH9.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (CD8) Monoclonal Antibody, Unconjugated (TMAB-00391) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded (human colon); Antigen retrieval by boiling in EDTA buffer (pH9.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (CD8) Monoclonal Antibody, Unconjugated (TMAB-00391) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 3. 25 μg total protein per Lane of various lysates probed with CD8 monoclonal antibody, unconjugated (TMAB-00391) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min. 4. Paraformaldehyde-fixed, paraffin embedded (human squamous cell lung cancer); Antigen retrieval by boiling in EDTA buffer (pH9.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (CD8) Monoclonal Antibody, Unconjugated (TMAB-00391) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 5. Paraformaldehyde-fixed, paraffin embedded (human colon cancer); Antigen retrieval by boiling in EDTA buffer (pH9.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (CD8) Monoclonal Antibody, Unconjugated (TMAB-00391) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 6. Paraformaldehyde-fixed, paraffin embedded (human spleen); Antigen retrieval by boiling in EDTA buffer (pH9.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (CD8) Monoclonal Antibody, Unconjugated (TMAB-00391) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Subcellular Localization | Membrane; Single-pass type I membrane protein. |
| Construction | Recombinant Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | PBS, Glycerol, BSA. |
| Research Background | The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen acts as a coreceptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell in the context of class I MHC molecules. The coreceptor functions as either a homodimer composed of two alpha chains or as a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 alpha chain. Multiple transcript variants encoding different isoforms have been found for this gene. The major protein isoforms of this gene differ by the presence or absence of a transmembrane domain and thus differ in being a membrane-anchored or secreted protein. [provided by RefSeq, May 2020] |
| Immunogen | KLH conjugated synthetic peptide: human CD8 |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | Cytotoxic Cells,CD,CD markers |
| Molecular Weight | Theoretical: 27 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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