Anti-CD8 alpha Antibody (9O552) is a Rabbit antibody targeting CD8 alpha. Anti-CD8 alpha Antibody (9O552) can be used in IF,IHC-Fr,IHC-P,WB.

IgG

9O552

Human

1. Paraformaldehyde-fixed, paraffin embedded (human tonsil); Antigen retrieval by boiling in EDTA buffer (pH9.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (CD8) Monoclonal Antibody, Unconjugated (TMAB-00391) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded (human colon); Antigen retrieval by boiling in EDTA buffer (pH9.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (CD8) Monoclonal Antibody, Unconjugated (TMAB-00391) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
3. 25 μg total protein per Lane of various lysates probed with CD8 monoclonal antibody, unconjugated (TMAB-00391) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min.
4. Paraformaldehyde-fixed, paraffin embedded (human squamous cell lung cancer); Antigen retrieval by boiling in EDTA buffer (pH9.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (CD8) Monoclonal Antibody, Unconjugated (TMAB-00391) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded (human colon cancer); Antigen retrieval by boiling in EDTA buffer (pH9.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (CD8) Monoclonal Antibody, Unconjugated (TMAB-00391) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded (human spleen); Antigen retrieval by boiling in EDTA buffer (pH9.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (CD8) Monoclonal Antibody, Unconjugated (TMAB-00391) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Monoclonal

KLH conjugated synthetic peptide: human CD8

Human

CD8A

T-cell surface glycoprotein CD8 alpha chain

Cytotoxic Cells,CD,CD markers

Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.