Anti-CD79b Antibody (2Q682) is a Rabbit antibody targeting CD79b. Anti-CD79b Antibody (2Q682) can be used in WB,ICC/IF,FCM.

IgG

2Q682

Human

1. The Ramos (H) cells were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.). Primary Antibody (green): Rabbit Anti-CD79b antibody (TMAB-04070): 1 μg/10^6 cells; Secondary Antibody (white blue): Goat anti-Rabbit IgG-FITC: 1 μg/test. Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.
2. (UPPER PANEL) 4% Paraformaldehyde-fixed Ramos (H) cell; Triton X-100 at r. T. for 20 min; Antibody incubation with (CD79b) monoclonal Antibody, unconjugated (TMAB-04070) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
(LOWER PANEL) MCF-7/Knockout Sample (H) cell as Negative Control. The experimental procedure was as same as above.
3. 25 μg total protein per lane of various lysates (see on figure) probed with CD79b monoclonal antibody, unconjugated (TMAB-04070) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.

WB: 1:1000-5000; ICC/IF: 1:100-500; FCM: 1:100-200

Monoclonal

A synthesized peptide: human CD79b

Human

CD79B

B-cell antigen receptor complex-associated protein beta chain

Required in cooperation with CD79A for initiation of the signal transduction cascade activated by the B-cell antigen receptor complex (BCR) which leads to internalization of the complex, trafficking to late endosomes and antigen presentation. Enhances phosphorylation of CD79A, possibly by recruiting kinases which phosphorylate CD79A or by recruiting proteins which bind to CD79A and protect it from dephosphorylation.