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Anti-CD75 Polyclonal Antibody

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Catalog No. TMAB-04067

Anti-CD75 Polyclonal Antibody is a Rabbit antibody targeting CD75. Anti-CD75 Polyclonal Antibody can be used in WB.

Anti-CD75 Polyclonal Antibody

Anti-CD75 Polyclonal Antibody

Copy Product Info
😃Good
Catalog No. TMAB-04067
Anti-CD75 Polyclonal Antibody is a Rabbit antibody targeting CD75. Anti-CD75 Polyclonal Antibody can be used in WB.
Pack SizePriceUSA WarehouseGlobal WarehouseQuantity
50 μL$2237-10 days7-10 days
100 μL$3717-10 days7-10 days
200 μL$5277-10 days7-10 days
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction

Bioactivity
Description
Anti-CD75 Polyclonal Antibody is a Rabbit antibody targeting CD75. Anti-CD75 Polyclonal Antibody can be used in WB.
Ig Type
IgG
Reactivity
Human,Mouse,Rat (predicted:Dog,Pig,Cow,Horse)
Verified Activity
1. Sample:
MCF-7 (Human) Cell Lysate at 30 μg
A549 (Human) Cell Lysate at 30 μg
A431 (Human) Cell Lysate at 30 μg
Siha (Human) Cell Lysate at 30 μg
Primary: Anti-CD75 (TMAB-04067) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 47 kD
Observed band size: 48 kD
2. Sample:
Liver (Mouse) Lysate at 40 μg
Primary: Anti-CD75 (TMAB-04067) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 47 kD
Observed band size: 48 kD
3. 25 μg total protein per lane of various lysates (see on figure) probed with CD75 polyclonal antibody, unconjugated (TMAB-04067) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
Application
Recommended Dose
WB: 1:500-2000
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationGolgi apparatus, Golgi stack membrane; Single-pass type II membrane protein. Secreted. Note=Membrane-bound form in trans cisternae of Golgi. Secreted into the body fluid.
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundThe protein encoded by this gene is a type II membrane protein that catalyzes the transfer of sialic acid from CMP sialic acid to galactose-containing substrates. The encoded protein, which is normally found in the Golgi apparatus but which can be proteolytically processed to a soluble form, is involved in the generation of the cell surface carbohydrate determinants and differentiation antigens HB6, CDw75, and CD76. This protein is a member of glycosyltransferase family 29. Three transcript variants encoding two different isoforms have been found for this gene.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human CD75
Antigen Species
Human
Gene Name
ST6GAL1
Gene ID
Protein Name
beta-galactoside alpha-2, 6-sialyltransferase 1
Uniprot ID
Function
Transfers sialic acid from the donor of substrate CMP-sialic acid to galactose containing acceptor substrates.
Chemical Properties
Molecular WeightTheoretical: 47 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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