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Anti-CD74 Antibody (3H62)
😃Good
Catalog No. TMAY-01630
Alias Ii, Ia-γ, Ia-GAMMA, HLADG, DHLAG, CLIP, CD74 molecule, major histocompatibility complex, class II invariant chain
Anti-CD74 Antibody (3H62) is a Mouse antibody targeting CD74. Anti-CD74 Antibody (3H62) can be used in IHC-P,FCM.
Anti-CD74 Antibody (3H62)
Anti-CD74 Antibody (3H62)
😃Good
Catalog No. TMAY-01630Alias Ii, Ia-γ, Ia-GAMMA, HLADG, DHLAG, CLIP, CD74 molecule, major histocompatibility complex, class II invariant chain
Anti-CD74 Antibody (3H62) is a Mouse antibody targeting CD74. Anti-CD74 Antibody (3H62) can be used in IHC-P,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 100 μL | $199 | 7-10 days |
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Antigen Details
Chemical Properties
| Description | Anti-CD74 Antibody (3H62) is a Mouse antibody targeting CD74. Anti-CD74 Antibody (3H62) can be used in IHC-P,FCM. |
| Alias | Ii, Ia-γ, Ia-GAMMA, HLADG, DHLAG, CLIP, CD74 molecule, major histocompatibility complex, class II invariant chain |
| Ig Type | Monoclonal Mouse IgG1 |
| Clone | 3H62 |
| Reactivity | Human |
| Verified Activity | 1. Immunochemical staining of human CD74 in human tonsil with mouse monoclonal antibody (1:1000, formalin-fixed paraffin embedded sections). 2. Immunochemical staining of human CD74 in human colon carcinoma with mouse monoclonal antibody (1:1000, formalin-fixed paraffin embedded sections). 3. Immunochemical staining of human CD74 in human appendix with mouse monoclonal antibody (1:1000, formalin-fixed paraffin embedded sections). 4. Flow cytometric analysis of Human CD74 expression on human whole blood lymphocytes. Cells were stained with purified anti-Human CD74, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. |
| Application | IHC-P,FCM |
| Recommended Dose | IHC-P: 1:500-1:2000; FCM: 1:25-1:100 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD74 (rh CD74; TMPY-01199; NP_004346.1; Gln73-Met232). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | The cluster of differentiation (CD) system is commonly used as cell markers in Immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD74, also known as HLA class2 histocompatibility antigen gamma chain and HLA-DR antigens-associated invariant chain, is a polypeptide involved in the formation and transport of MHC class2 protein. CD74 is expressed by B cells, macrophages, and Reed-Sternberg cells. When MHC class 2 protein was in the rough ER, its peptide-binding cleft was blocked by CD74 to prevent it from interacting with the endogenous peptides. CD74 also serves to facilitate MHC class2's export from ER. |
| Conjucates | Unconjugated |
| Others Formats | APC/PE |
| Antibody Types Available | 3 |
| Immunogen | Recombinant Human CD74 protein (TMPY-01199) |
| Antigen Species | Human |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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