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Anti-CD73 Polyclonal Antibody

😃Good
Catalog No. TMAB-00386

Anti-CD73 Polyclonal Antibody is a Rabbit antibody targeting CD73. Anti-CD73 Polyclonal Antibody can be used in WB,FCM.

Anti-CD73 Polyclonal Antibody

Anti-CD73 Polyclonal Antibody

😃Good
Catalog No. TMAB-00386
Anti-CD73 Polyclonal Antibody is a Rabbit antibody targeting CD73. Anti-CD73 Polyclonal Antibody can be used in WB,FCM.
Pack SizePriceAvailabilityQuantity
50 μL$222 7-10 days
100 μL$373 7-10 days
200 μL$528 7-10 days
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Product Introduction

Bioactivity
Description
Anti-CD73 Polyclonal Antibody is a Rabbit antibody targeting CD73. Anti-CD73 Polyclonal Antibody can be used in WB,FCM.
Ig Type
IgG
Reactivity
Human,Mouse (predicted:Rat,Dog,Pig,Cow,Rabbit,Sheep,GuineaPig)
Verified Activity
1. Sample:
Lane 1: A431 (Human) Cell Lysate at 30 μg
Lane 2: A549 (Human) Cell Lysate at 30 μg
Lane 3: HepG2 (Human) Cell Lysate at 30 μg
Lane 4: U251 (Human) Cell Lysate at 30 μg
Primary: Anti-CD73 (TMAB-00386) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 63/71 kDa
Observed band size: 65 kDa
2. Sample: Kidney (mouse) l Lysate at 40 μg
Primary: Anti-CD73 (TMAB-00386) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 63 kDa
Observed band size: 65 kDa
3. Sample: HepG2 (human) cell Lysate at 40 μg
Primary: Anti-CD73 (TMAB-00386) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 63 kDa
Observed band size: 63 kDa
4. Blank control (blue line): Raji (blue).
Primary Antibody (green line): Rabbit Anti-Phospho-c-Fos (Ser32) antibody (TMAB-00386)
Dilution: 1 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE
Dilution: 1 μg/test.
Application
WB,FCM
Recommended Dose
WB: 1:500-2000; FCM: 1μg/Test
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationCell membrane.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundThe protein encoded by this gene is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. The encoded protein is used as a determinant of lymphocyte differentiation. Defects in this gene can lead to the calcification of joints and arteries. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2011]
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human CD73/NT5
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Associated Proteins,Leukocyte recruitment
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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