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Anti-CD73 Polyclonal Antibody
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Catalog No. TMAB-00386
Alias NTE, NT5, NT, eNT, eN, E5NT, CD73, CALJA, 5'-nucleotidase, ecto (CD73)
Anti-CD73 Polyclonal Antibody is a Rabbit antibody targeting CD73. Anti-CD73 Polyclonal Antibody can be used in WB,FCM.
Anti-CD73 Polyclonal Antibody
Anti-CD73 Polyclonal Antibody
😃Good
Catalog No. TMAB-00386Alias NTE, NT5, NT, eNT, eN, E5NT, CD73, CALJA, 5'-nucleotidase, ecto (CD73)
Anti-CD73 Polyclonal Antibody is a Rabbit antibody targeting CD73. Anti-CD73 Polyclonal Antibody can be used in WB,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $222 | 7-10 days | |
| 100 μL | $373 | 7-10 days | |
| 200 μL | $528 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-CD73 Polyclonal Antibody is a Rabbit antibody targeting CD73. Anti-CD73 Polyclonal Antibody can be used in WB,FCM. |
| Alias | NTE, NT5, NT, eNT, eN, E5NT, CD73, CALJA, 5'-nucleotidase, ecto (CD73) |
| Ig Type | IgG |
| Reactivity | Human,Mouse (predicted:Rat,Dog,Pig,Cow,Rabbit,Sheep,GuineaPig) |
| Verified Activity | 1. Sample: Lane 1: A431 (Human) Cell Lysate at 30 μg Lane 2: A549 (Human) Cell Lysate at 30 μg Lane 3: HepG2 (Human) Cell Lysate at 30 μg Lane 4: U251 (Human) Cell Lysate at 30 μg Primary: Anti-CD73 (TMAB-00386) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 63/71 kDa Observed band size: 65 kDa 2. Sample: Kidney (mouse) l Lysate at 40 μg Primary: Anti-CD73 (TMAB-00386) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 63 kDa Observed band size: 65 kDa 3. Sample: HepG2 (human) cell Lysate at 40 μg Primary: Anti-CD73 (TMAB-00386) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 63 kDa Observed band size: 63 kDa 4. Blank control (blue line): Raji (blue). Primary Antibody (green line): Rabbit Anti-Phospho-c-Fos (Ser32) antibody (TMAB-00386) Dilution: 1 μg/10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE Dilution: 1 μg/test. |
| Application | |
| Recommended Dose | WB: 1:500-2000; FCM: 1μg/Test |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cell membrane. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | The protein encoded by this gene is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. The encoded protein is used as a determinant of lymphocyte differentiation. Defects in this gene can lead to the calcification of joints and arteries. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2011] |
| Immunogen | KLH conjugated synthetic peptide: human CD73/NT5 |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | Associated Proteins,Leukocyte recruitment |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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