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Anti-CD73 Antibody (9U110)

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Catalog No. TMAY-01317
Alias NTE, NT5, NT, eNT, eN, E5NT, CD73, CALJA, 5'-nucleotidase, ecto (CD73)

Anti-CD73 Antibody (9U110) is a Mouse antibody targeting CD73. Anti-CD73 Antibody (9U110) can be used in FCM.

Anti-CD73 Antibody (9U110)

Anti-CD73 Antibody (9U110)

😃Good
Catalog No. TMAY-01317Alias NTE, NT5, NT, eNT, eN, E5NT, CD73, CALJA, 5'-nucleotidase, ecto (CD73)
Anti-CD73 Antibody (9U110) is a Mouse antibody targeting CD73. Anti-CD73 Antibody (9U110) can be used in FCM.
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100 μL$199 7-10 days
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Product Introduction

Bioactivity
Description
Anti-CD73 Antibody (9U110) is a Mouse antibody targeting CD73. Anti-CD73 Antibody (9U110) can be used in FCM.
AliasNTE, NT5, NT, eNT, eN, E5NT, CD73, CALJA, 5'-nucleotidase, ecto (CD73)
Ig Type
Monoclonal Mouse IgG1
Clone
9U110
Reactivity
Human
Verified Activity
Flow cytometric analysis of Human NT5E(CD73) expression on human whole blood lymphocytes. Cells were stained with purified anti-Human NT5E(CD73), then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes.
Application
FCM
Recommended Dose
FCM: 1:25-1:100
Antibody Type
Monoclonal
Host SpeciesMouse
ConstructionThis antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD73 / NT5E (rh CD73 / NT5E; TMPY-01851; NP_002517.1; Met1-Lys547). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
PurificationProtein A
AppearanceLiquid
Formulation0.2 μm filtered solution in PBS
Research Background5'-nucleotidase, also known as NT5E, NTE, and CD73, is a cell membrane protein that belongs to the 5'-nucleotidase family. CD73 is a glycosylphosphatidylinositol (GPI) anchored purine salvage enzyme expressed on the surface of human T and B lymphocytes. CD73 catalyzes the conversion of purine and pyrimidine ribo- and deoxyribonucleoside monophosphates to the corresponding nucleosides. CD73 serves as a costimulatory molecule in activating T cells. CD73 generated adenosine functions in cell signaling in many physiologic systems, including intestinal epithelium, ischemic myocardium, and cholinergic synapses. CD73 might mediate lymphocyte-stromal cell interactions or condition the local microenvironment to facilitate lymphocyte development and/or function. In CD73-depleted cells, surface levels of the leukocyte adhesion molecules ICAM-1, VCAM-1, and E-selectin increase. CD73 produces extracellular adenosine, which then acts on G protein-coupled purinergic receptors to induce cellular responses. CD73 has also been reported to regulate the expression of pro-inflammatory molecules in mouse endothelium.
Related Conjugates and Formulations
Conjucates
Unconjugated
Others Formats
FITC/PE
Antibody Types Available
3
Antigen Details
Immunogen
Recombinant Human CD73 / NT5E Protein (TMPY-01851)
Antigen Species
Human
Biology Area
Phosphatases and Regulators
Chemical Properties
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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