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Anti-CD7 Antibody (4Z579)

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Catalog No. TMAY-01495
Alias CD7 molecule

Anti-CD7 Antibody (4Z579) is a Rabbit antibody targeting CD7. Anti-CD7 Antibody (4Z579) can be used in WB,IP.

Anti-CD7 Antibody (4Z579)

Anti-CD7 Antibody (4Z579)

😃Good
Catalog No. TMAY-01495Alias CD7 molecule
Anti-CD7 Antibody (4Z579) is a Rabbit antibody targeting CD7. Anti-CD7 Antibody (4Z579) can be used in WB,IP.
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100 μL$269 7-10 days
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Product Introduction

Bioactivity
Description
Antibody Type: Rabbit Monoclonal

Application: WB, IP

Reactivity: Human
AliasCD7 molecule
Ig Type
Rabbit IgG
Clone
4Z579
Reactivity
Human
Verified Activity
1. Anti-CD7 rabbit monoclonal antibody at 1:500 dilution.
-Lane A: Jurkat Whole Cell lysate.
-Lysates/proteins at 30 μg per lane.
-Secondary
-Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.
-Developed using the Odyssey technique.
-Performed under reducing conditions.
-Predicted band size:25 kDa.
-Observed band size:37 kDa.
2. CD7 was immunoprecipitated using:
-Lane A:0.5 mg Jurkat Whole Cell Lysate.
-2 µL anti-CD7 rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose.
-Primary antibody:
-Anti-CD7 rabbit monoclonal antibody, at 1:200 dilution.
-Secondary antibody:
-Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution.
-Developed using the odyssey technique.
-Performed under reducing conditions.
-Predicted band size: 34 kDa.
-Observed band size: 34 kDa
Application
WB,IP
Recommended Dose
WB: 1:500-1:2000; IP: 4-6 μL/mg of lysate
Antibody Type
Monoclonal
Host SpeciesRabbit
ConstructionThis antibody was obtained from a rabbit immunized with purified, recombinant Human CD7 (rh CD7; TMPY-01195; NP_006128.1; Met1-Pro180).
PurificationProtein A
AppearanceLiquid
Formulation0.2 μm filtered solution in PBS
Research BackgroundThe cluster of differentiation (CD) system is commonly used as cell markers in Immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD7 is a transmembrane protein that is a member of the immunoglobulin superfamily. This protein is found on thymocytes and mature T cells. It plays an essential role in T-cell interactions and also in T-cell / B-cell interaction during early lymphoid development.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Protein: Human CD7 protein (TMPY-01195)
Antigen Species
Human
Chemical Properties
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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