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Anti-CD7 Antibody (4Z579) is a Rabbit antibody targeting CD7. Anti-CD7 Antibody (4Z579) can be used in WB,IP.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
100 μL | $269 | 7-10 days |
Description | Antibody Type: Rabbit Monoclonal Application: WB, IP Reactivity: Human |
Alias | CD7 molecule |
Ig Type | Rabbit IgG |
Clone | 4Z579 |
Reactivity | Human |
Verified Activity | 1. Anti-CD7 rabbit monoclonal antibody at 1:500 dilution. -Lane A: Jurkat Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:25 kDa. -Observed band size:37 kDa. 2. CD7 was immunoprecipitated using: -Lane A:0.5 mg Jurkat Whole Cell Lysate. -2 µL anti-CD7 rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose. -Primary antibody: -Anti-CD7 rabbit monoclonal antibody, at 1:200 dilution. -Secondary antibody: -Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. -Developed using the odyssey technique. -Performed under reducing conditions. -Predicted band size: 34 kDa. -Observed band size: 34 kDa |
Application | WB,IP |
Recommended Dose | WB: 1:500-1:2000; IP: 4-6 μL/mg of lysate |
Antibody Type | Monoclonal |
Host Species | Rabbit |
Construction | This antibody was obtained from a rabbit immunized with purified, recombinant Human CD7 (rh CD7; TMPY-01195; NP_006128.1; Met1-Pro180). |
Purification | Protein A |
Appearance | Liquid |
Formulation | 0.2 μm filtered solution in PBS |
Research Background | The cluster of differentiation (CD) system is commonly used as cell markers in Immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD7 is a transmembrane protein that is a member of the immunoglobulin superfamily. This protein is found on thymocytes and mature T cells. It plays an essential role in T-cell interactions and also in T-cell / B-cell interaction during early lymphoid development. |
Conjucates | Unconjugated |
Immunogen | Recombinant Protein: Human CD7 protein (TMPY-01195) |
Antigen Species | Human |
Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
Transport | Shipping with blue ice. |
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