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Anti-CD63 Antibody (7S689)
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Catalog No. TMAB-00378
Anti-CD63 Antibody (7S689) is a Rabbit antibody targeting CD63. Anti-CD63 Antibody (7S689) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.
Anti-CD63 Antibody (7S689)
Anti-CD63 Antibody (7S689)
😃Good
Catalog No. TMAB-00378
Anti-CD63 Antibody (7S689) is a Rabbit antibody targeting CD63. Anti-CD63 Antibody (7S689) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 25 μL | $154 | 7-10 days | |
| 50 μL | $271 | 7-10 days | |
| 100 μL | $489 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Antibody Type: Recombinant Rabbit Monoclonal Application: WB,IHC-P,IHC-Fr,ICC/IF,IF Reactivity: Human |
| Ig Type | IgG |
| Clone | 7S689 |
| Reactivity | Human |
| Verified Activity | 1. 25 μg total protein per Lane of various lysates probed with CD63 monoclonal antibody, unconjugated (TMAB-00378) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min. 2. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD63 Monoclonal Antibody, Unconjugated (TMAB-00378) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded Human Lymph; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD63 Monoclonal Antibody, Unconjugated (TMAB-00378) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD63 Monoclonal Antibody, Unconjugated (TMAB-00378) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 5. 4% Paraformaldehyde-fixed A375 (H) cell; Triton X-100 at RT for 20 min; Antibody incubation with (CD63) monoclonal Antibody, unconjugated (TMAB-00378) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green ) at 37°C for 90 min, DAPI (blue) was used to stain the cell nucleus. PBS instead of the primary antibody was used as the blank control. |
| Application | WB,IHC-P,IHC-Fr,ICC/IF,IF |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:50-200; IF: 1:100-500 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cell membrane; Multi-pass membrane protein. Lysosome membrane; Multi-pass membrane protein. Late endosome membrane; Multi-pass membrane protein. Note=Also found in Weibel-Palade bodies of endothelial cells. Located in platelet dense granules. |
| Construction | Recombinant Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. The encoded protein is a cell surface glycoprotein that is known to complex with integrins. It may function as a blood platelet activation marker. Deficiency of this protein is associated with Hermansky-Pudlak syndrome. Also this gene has been associated with tumor progression. Alternative splicing results in multiple transcript variants encoding different protein isoforms. [provided by RefSeq, Apr 2012] |
| Immunogen | A synthesized peptide: human CD63 |
| Antigen Species | Human |
| Gene ID | 967 |
| Uniprot ID | |
| Biology Area | Cell adhesion,Tumor antigens,Platelets,Platelets,Tumor Associated |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
Sci Citations
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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