Anti-CD62p Antibody (1S872) is a Mouse antibody targeting CD62p. Anti-CD62p Antibody (1S872) can be used in IHC-P,IHC-Fr,IF,FCM,ICC/IF.

IgG1

1S872

Human

1. Paraformaldehyde-fixed, paraffin embedded Human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD62p Monoclonal Antibody, Unconjugated (TMAB-04037) at 1: 200 overnight at 4°C, followed by conjugation to the AffiniPure Goat Anti-Mouse IgG H&L-HRP and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded Human tonsils; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD62p Monoclonal Antibody, Unconjugated (TMAB-04037) at 1: 200 overnight at 4°C, followed by conjugation to the AffiniPure Goat Anti-Mouse IgG H&L-HRP and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Human lungs; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD62p Monoclonal Antibody, Unconjugated (TMAB-04037) at 1: 200 overnight at 4°C, followed by conjugation to the AffiniPure Goat Anti-Mouse IgG H&L-HRP and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Human colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD62p Monoclonal Antibody, Unconjugated (TMAB-04037) at 1: 200 overnight at 4°C, followed by conjugation to the AffiniPure Goat Anti-Mouse IgG H&L-HRP and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Human spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD62p Monoclonal Antibody, Unconjugated (TMAB-04037) at 1: 200 overnight at 4°C, followed by conjugation to the AffiniPure Goat Anti-Mouse IgG H&L-HRP and DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded Human esophagus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD62p Monoclonal Antibody, Unconjugated (TMAB-04037) at 1: 200 overnight at 4°C, followed by conjugation to the AffiniPure Goat Anti-Mouse IgG H&L-HRP and DAB staining.
7. Paraformaldehyde-fixed, paraffin embedded Human Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD62p Monoclonal Antibody, Unconjugated (TMAB-04037) at 1: 200 overnight at 4°C, followed by conjugation to the AffiniPure Goat Anti-Mouse IgG H&L-HRP and DAB staining.
8. Paraformaldehyde-fixed, paraffin embedded Human esophageal cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD62p Monoclonal Antibody, Unconjugated (TMAB-04037) at 1: 200 overnight at 4°C, followed by conjugation to the AffiniPure Goat Anti-Mouse IgG H&L-HRP and DAB staining.
9. The Hel92.1.7 (H) cells were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.). Primary Antibody (green): Mouse Anti-CD62p antibody (TMAB-04037): 1 μg/10^6 cells; Secondary Antibody (white blue): Goat anti-Mouse IgG-BF488: 1 μg/test. Blank control (black): PBS. Acquisition of 20,000 events was performed.
10. 4% Paraformaldehyde-fixed Hel 92.1.7 (H) cell; Antibody incubation with (CD62p) monoclonal Antibody, unconjugated (TMAB-04037) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Mouse IgG antibody (green) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1μg/Test; ICC/IF: 1:50-200

Monoclonal

Recombinant Protein: human P-Selectin

Human

SELP

P-selectin

Ca(2+)-dependent receptor for myeloid cells that binds to carbohydrates on neutrophils and monocytes. Mediates the interaction of activated endothelial cells or platelets with leukocytes. The ligand recognized is sialyl-Lewis X. Mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with PSGL1.