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Anti-CD5L Polyclonal Antibody
😃Good
Catalog No. TMAB-00376
Alias SP-α, Spα, SP-ALPHA, Spalpha, PRO229, CD5 molecule-like, API6, AIM
Anti-CD5L Polyclonal Antibody is a Rabbit antibody targeting CD5L. Anti-CD5L Polyclonal Antibody can be used in IF, IHC-Fr, IHC-P.
Anti-CD5L Polyclonal Antibody
Anti-CD5L Polyclonal Antibody
😃Good
Catalog No. TMAB-00376Alias SP-α, Spα, SP-ALPHA, Spalpha, PRO229, CD5 molecule-like, API6, AIM
Anti-CD5L Polyclonal Antibody is a Rabbit antibody targeting CD5L. Anti-CD5L Polyclonal Antibody can be used in IF, IHC-Fr, IHC-P.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $220 | 7-10 days | 7-10 days | |
| 100 μL | $372 | 7-10 days | 7-10 days | |
| 200 μL | $529 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-CD5L Polyclonal Antibody is a Rabbit antibody targeting CD5L. Anti-CD5L Polyclonal Antibody can be used in IF, IHC-Fr, IHC-P. |
| Synonyms | SP-α, Spα, SP-ALPHA, Spalpha, PRO229, CD5 molecule-like, API6, AIM |
| Ig Type | IgG |
| Reactivity | Mouse,Rat (predicted:Human) |
| Verified Activity | 1. Tissue/cell: mouse lymphoma tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-CD5L/Api6 Polyclonal Antibody, Unconjugated (TMAB-00376) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 2. Paraformaldehyde-fixed, paraffin embedded (rat spleen tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CD5L) Polyclonal Antibody, Unconjugated (TMAB-00376) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining.   |
| Application | |
| Recommended Dose | IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Secreted. |
| Tissue Specificity | Expressed in spleen, lymph node, thymus, bone marrow, and fetal liver, but not in non-lymphoid tissues. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1 mg/mL |
| Research Background | CD5L is a member of the scavenger receptor cysteine-rich domain superfamily (SRCR-SF) initially identified as an inducible cell surface ligand of CD5. It was shown that CD5L functions in the thymus as the inducer of resistance to apoptosis within CD4+/CD8+ thymocytes and as the supporter of the viability of these cells before thymic selection. CD5L was also shown to support macrophage survival and enhance their phagocytic function. More recent experiments using recombinant CD5L significantly inhibited apoptosis of NKT and T cells obtained from C. parvum-stimulated livers in vitro, suggesting that CD5L functions to induce resistance to apoptosis in these cells and supports host defense against inflammation during infection. Expressed in spleen, lymph node, thymus, bone marrow, and fetal liver, but not in non-lymphoid tissues. |
| Immunogen | KLH conjugated synthetic peptide: human CD5L |
| Antigen Species | Human |
| Gene Name | CD5L |
| Gene ID | |
| Protein Name | CD5 antigen-like |
| Uniprot ID | |
| Biology Area | Survivin / IAPs |
| Function | May play a role in the regulation of the immune system. Seems to play a role as an inhibitor of apoptosis. |
| Molecular Weight | Theoretical: 36 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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