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Anti-CD57 Antibody (4O28)

Catalog No. TMAB-04030 Copy Product Info
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Anti-CD57 Antibody (4O28) is a Mouse antibody targeting CD57. Anti-CD57 Antibody (4O28) can be used in IHC-P,IHC-Fr,IF.

Anti-CD57 Antibody (4O28)

Copy Product Info
🥰Excellent
Catalog No. TMAB-04030

Anti-CD57 Antibody (4O28) is a Mouse antibody targeting CD57. Anti-CD57 Antibody (4O28) can be used in IHC-P,IHC-Fr,IF.

Anti-CD57 Antibody
(4O28)
Pack SizePriceUSA StockGlobal StockQuantity
50 μL$2217-10 days7-10 days
100 μL$3727-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-CD57 Antibody (4O28) is a Mouse antibody targeting CD57. Anti-CD57 Antibody (4O28) can be used in IHC-P,IHC-Fr,IF.
Ig Type
IgG1, k
Clone
4O28
Reactivity
Human
Verified Activity
1. Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD57 Monoclonal Antibody, Unconjugated (TMAB-04030) at 1:100 overnight at 4°C, followed by conjugation to the AffiniPure Goat Anti-Mouse IgG H&L-HRP and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded Human Chronic tonsillitis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD57 Monoclonal Antibody, Unconjugated (TMAB-04030) at 1:100 overnight at 4°C, followed by conjugation to the AffiniPure Goat Anti-Mouse IgG H&L-HRP and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD57 Monoclonal Antibody, Unconjugated (TMAB-04030) at 1:100 overnight at 4°C, followed by conjugation to the AffiniPure Goat Anti-Mouse IgG H&L-HRP and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Human Prostate Tumor; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD57 Monoclonal Antibody, Unconjugated (TMAB-04030) at 1:100 overnight at 4°C, followed by conjugation to the AffiniPure Goat Anti-Mouse IgG H&L-HRP and DAB staining.
Application
Recommended Dose
IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Monoclonal
Host SpeciesMouse
Subcellular LocalizationGolgi apparatus membrane; Single-pass type II membrane protein.
Tissue SpecificityMainly expressed in the brain.
ConstructionHybridoma Monoclonal Antibody
PurificationProtein G purified
AppearanceLyophilized or Liquid
FormulationPBS, Glycerol, BSA.
Concentration1mg/ml
Research BackgroundThe protein encoded by this gene is a member of the glucuronyltransferase gene family. These enzymes exhibit strict acceptor specificity, recognizing nonreducing terminal sugars and their anomeric linkages. This gene product functions as the key enzyme in a glucuronyl transfer reaction during the biosynthesis of the carbohydrate epitope HNK-1 (human natural killer-1, also known as CD57 and LEU7). Alternate transcriptional splice variants have been characterized. [provided by RefSeq, Jul 2008]
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human CD57
Antigen Species
Human
Gene Name
B3GAT1
Gene ID
Protein Name
Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1
Uniprot ID
Function
Involved in the biosynthesis of L2/HNK-1 carbohydrate epitope on glycoproteins. Can also play a role in glycosaminoglycan biosynthesis. Substrates include asialo-orosomucoid (ASOR), asialo-fetuin, and asialo-neural cell adhesion molecule. Requires sphingomyelin for activity: stearoyl-sphingomyelin was the most effective, followed by palmitoyl-sphingomyelin and lignoceroyl-sphingomyelin. Activity was demonstrated only for sphingomyelin with a saturated fatty acid and not for that with an unsaturated fatty acid, regardless of the length of the acyl group.
Chemical Properties
Molecular WeightTheoretical: 38 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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