Anti-CD53 Polyclonal Antibody is a Rabbit antibody targeting CD53. Anti-CD53 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human

1. Tissue/cell: human colon cancer; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-CD53 Polyclonal Antibody, Unconjugated (TMAB-04021) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
2. Sample:
Jurkat (Human) Cell Lysate at 30 μg
Molt-4 (Human) Cell Lysate at 30 μg
Primary: Anti-CD53 (TMAB-04021) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 24 kD
Observed band size: 24 kD
3. Sample:
Molt-4 (Human) Cell Lysate at 30 μg
Primary: Anti-CD53 (TMAB-04021) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 24 kD
Observed band size: 26 kD
4. Sample:
MOLT-4 (Human) Cell Lysate at 30 μg
Primary: Anti-CD53 (TMAB-04021) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 24 kD
Observed band size: 25 kD

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human CD53

Human

CD53

Leukocyte surface antigen CD53

Required for efficient formation of myofibers in regenerating muscle at the level of cell fusion. May be involved in growth regulation in hematopoietic cells (By similarity).