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Anti-CD50 Antibody (3V886)

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Catalog No. TMAB-04018

Anti-CD50 Antibody (3V886) is a Rabbit antibody targeting CD50. Anti-CD50 Antibody (3V886) can be used in IHC-P,IHC-Fr,ICC/IF,IF,WB.

Anti-CD50 Antibody (3V886)

Anti-CD50 Antibody (3V886)

Copy Product Info
😃Good
Catalog No. TMAB-04018
Anti-CD50 Antibody (3V886) is a Rabbit antibody targeting CD50. Anti-CD50 Antibody (3V886) can be used in IHC-P,IHC-Fr,ICC/IF,IF,WB.
Pack SizePriceUSA WarehouseGlobal WarehouseQuantity
25 μL$1517-10 days7-10 days
50 μL$2637-10 days7-10 days
100 μL$4727-10 days7-10 days
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction

Bioactivity
Description
Anti-CD50 Antibody (3V886) is a Rabbit antibody targeting CD50. Anti-CD50 Antibody (3V886) can be used in IHC-P,IHC-Fr,ICC/IF,IF,WB.
Ig Type
IgG
Clone
3V886
Reactivity
Human
Verified Activity
1. 25 μg total protein per lane of various lysates (see on figure) probed with CD50 monoclonal antibody, unconjugated (TMAB-04018) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
2. Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD50 Monoclonal Antibody, Unconjugated (TMAB-04018) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD50 Monoclonal Antibody, Unconjugated (TMAB-04018) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD50 Monoclonal Antibody, Unconjugated (TMAB-04018) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
Application
Recommended Dose
IHC-P: 1:50-200; IHC-Fr: 1:50-200; ICC/IF: 1:50-200; IF: 1:50-200; WB: 1:500-2000
Antibody Type
Monoclonal
Host SpeciesRabbit
Subcellular LocalizationMembrane; Single-pass type I membrane protein.
ConstructionRecombinant Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundThe protein encoded by this gene is a member of the intercellular adhesion molecule (ICAM) family. All ICAM proteins are type I transmembrane glycoproteins, contain 2-9 immunoglobulin-like C2-type domains, and bind to the leukocyte adhesion LFA-1 protein. This protein is constitutively and abundantly expressed by all leucocytes and may be the most important ligand for LFA-1 in the initiation of the immune response. It functions not only as an adhesion molecule, but also as a potent signalling molecule. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Feb 2016]
Antigen Details
Immunogen
A synthesized peptide: human ICAM 3
Gene Name
ICAM3
Gene ID
Protein Name
Intercellular adhesion molecule 3
Function
ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). ICAM3 is also a ligand for integrin alpha-D/beta-2.
Chemical Properties
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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