Anti-CD5 Antibody (3E812) is a Mouse antibody targeting CD5. Anti-CD5 Antibody (3E812) can be used in WB,IHC-P,IHC-Fr,IF,FCM.

IgG

3E812

Human

1. Blank control (black line): Jurkat.
Primary Antibody (green line): Mouse Anti-CD5 antibody (TMAB-04014)
Dilution: 1 μg/Test;
Secondary Antibody (white blue line): Goat anti-mouse IgG-AF488
Dilution: 0.5 μg/Test.
Isotype control (orange line): Normal Mouse IgG
Protocol
The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
2. Paraformaldehyde-fixed, paraffin embedded (human tonsil); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (CD5) Monoclonal Antibody, Unconjugated (TMAB-04014) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.
3. Negative sample: Paraformaldehyde-fixed, paraffin embedded (human skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Paraformaldehyde-fixed, paraffin embedded (human skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (CD5) Monoclonal Antibody, Unconjugated (TMAB-04014) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded (human lymphoid); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (CD5) Monoclonal Antibody, Unconjugated (TMAB-04014) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded (human spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (CD5) Monoclonal Antibody, Unconjugated (TMAB-04014) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.
6. Sample:
Lane 1: Human MOLT4 cell lysates
Lane 2: Human Raji cell lysates
Primary: Anti-CD5 (TMAB-04014) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 55 kDa
Observed band size: 60 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1μg/Test

Monoclonal

KLH conjugated synthetic peptide: human CD5

Human

CD5

T-cell surface glycoprotein CD5

May act as a receptor in regulating T-cell proliferation.