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Anti-CD44 Polyclonal Antibody 2
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Catalog No. TMAB-00370
Anti-CD44 Polyclonal Antibody 2 is a Rabbit antibody targeting CD44. Anti-CD44 Polyclonal Antibody 2 can be used in FCM.
Anti-CD44 Polyclonal Antibody 2
Anti-CD44 Polyclonal Antibody 2
😃Good
Catalog No. TMAB-00370
Anti-CD44 Polyclonal Antibody 2 is a Rabbit antibody targeting CD44. Anti-CD44 Polyclonal Antibody 2 can be used in FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $221 | 7-10 days | |
| 100 μL | $373 | 7-10 days | |
| 200 μL | $527 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Antibody Type: Rabbit Polyclonal Application: FCM Reactivity: Human,Mouse (predicted:Rat,Chicken,Pig,Cow,Rabbit) |
| Ig Type | IgG |
| Reactivity | Human,Mouse (predicted:Rat,Chicken,Pig,Cow,Rabbit) |
| Verified Activity | 1. Blank control (blue): U251 (fixed with Ice-cold 70% ethanol overnight at 4°C). Primary Antibody: Rabbit Anti-CD44 antibody (TMAB-00370,Green); Dilution: 1 μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit Igg (orange), used under the same conditions; Secondary Antibody: Goat anti-rabbit IgG-FITC (white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. 2. Blank control: HELA. Primary Antibody (green line): Rabbit Anti-CD44 antibody (TMAB-00370) Dilution: 1 μg/10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF647 Dilution: 1 μg/test. Protocol The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. |
| Application | FCM |
| Recommended Dose | FCM: 1μg/Test |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Membrane; Single-pass type I membrane protein. Note=Colocalizes with actin in membrane protrusions at wounding edges. |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | The protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis. [provided by RefSeq, Jul 2008]. |
| Immunogen | KLH conjugated synthetic peptide: rat CD44v6 |
| Antigen Species | Rat |
| Gene ID | 25406 |
| Uniprot ID | |
| Biology Area | Tumor biomarkers,CD markers,Adhesion,CD markers ELISA kits,T Lymphocytic Lineage,Dendritic Cell Lineage,Neutrophil Lineage,Surface Molecules |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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