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Anti-CD44 Antibody (7W954)

😃Good
Catalog No. TMAY-02283
Alias Pgp1, MIC4, MDU3, MDU2, MC56, LHR, IN, HUTCH-I, HCELL, ECMR-III, CSPG8, CDW44, CD44 molecule (Indian blood group)

Anti-CD44 Antibody (7W954) is a Mouse antibody targeting CD44. Anti-CD44 Antibody (7W954) can be used in FCM,ICC/IF.

Anti-CD44 Antibody (7W954)

Anti-CD44 Antibody (7W954)

😃Good
Catalog No. TMAY-02283Alias Pgp1, MIC4, MDU3, MDU2, MC56, LHR, IN, HUTCH-I, HCELL, ECMR-III, CSPG8, CDW44, CD44 molecule (Indian blood group)
Anti-CD44 Antibody (7W954) is a Mouse antibody targeting CD44. Anti-CD44 Antibody (7W954) can be used in FCM,ICC/IF.
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100 μL$198 7-10 days
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Product Introduction

Bioactivity
Description
Anti-CD44 Antibody (7W954) is a Mouse antibody targeting CD44. Anti-CD44 Antibody (7W954) can be used in FCM,ICC/IF.
AliasPgp1, MIC4, MDU3, MDU2, MC56, LHR, IN, HUTCH-I, HCELL, ECMR-III, CSPG8, CDW44, CD44 molecule (Indian blood group)
Ig Type
Monoclonal Mouse IgG1
Clone
7W954
Reactivity
Human
Verified Activity
1. Immunofluorescence staining of Human CD44 in Hela cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Mouse anti-Human CD44 monoclonal antibody (1:60) at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cell membrane and cytoplasm.

2. Flow cytometric analysis of Human CD44 expression on human peripheral blood lymphocytes. Cells were stained with purified Mouse anti-Human CD44, then a FITC-conjugated second step antibody. The histogram were derived from events with the forward and side light-scatter characteristics of viable lymphocytes.
Application
FCM,ICC/IF
Recommended Dose
ICC-IF: 1:20-1:100; FCM: 1:25-1:100
Antibody Type
Monoclonal
Host SpeciesMouse
ConstructionThis antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD44 (rh CD44; TMPY-02033; P16070-1; Met1-Pro220). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
PurificationProtein A
AppearanceLiquid
Formulation0.2 μm filtered solution in PBS
Research BackgroundCD44 is a type I transmembrane protein and a member of the cartilage link protein family. It is involved in cell-cell and cell-matrix interactions and signal transduction. Several CD44 ligands have been identified. The most extensively characterized ligand for CD44 is hyaluronan, a component of the extracellular matrix. CD44 protein is expressed on the majority of immune cells. The binding of CD44 to hyaluronan is induced on T lymphocytes after activation by antigen and on monocytes after stimulation by inflammatory agents. Under inflammatory conditions, CD44 on endothelial cells presents hyaluronan to CD44 on activated T lymphocytes and mediates a rolling interaction under flow conditions. Perturbations of the hyaluronan-CD44 interaction at the plasma membrane by various antagonists result in attenuation of receptor tyrosine kinase and transporter activities and inhibition of tumor progression in vivo. CD44 is known to interact with the ezrin family (ERM family) members and form a complex that plays diverse roles within both normal and abnormal cells, particularly cancer cells. CD44 and ezrin and their respective complex have properties suggesting that they may be important in the process of tumour-endothelium interactions, cell migrations, cell adhesion, tumour progression and metastasis.
Related Conjugates and Formulations
Conjucates
Unconjugated
Others Formats
APC/PerCP/FITC/PE
Antibody Types Available
5
Antigen Details
Immunogen
Recombinant Human CD44 protein (TMPY-02033)
Antigen Species
Human
Chemical Properties
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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