Anti-CD44 Antibody (6P561) is a Mouse antibody targeting CD44. Anti-CD44 Antibody (6P561) can be used in IHC-P,IHC-Fr,ICC/IF,IF,FCM.

Mouse IgG2a, k

6P561

Human

1. Blank control: Hela.
Primary Antibody (green line): Mouse Anti-human CD44 antibody (TMAB-03989)
Dilution: 1 ug/Test;
Secondary Antibody (white blue line) : Goat anti-Mouse IgG-AF488
Dilution: 0.5ug/Test.
Isotype control（orange line）：Normal Mouse IgG
Protocol
The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
2. Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (human CD44) monoclonal Antibody, Unconjugated (TMAB-03989) 1: 100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Mouse IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
3. Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (human CD44)) Monoclonal Antibody, Unconjugated (TMAB-03989) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded (human esophagus cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (human CD44)) Monoclonal Antibody, Unconjugated (TMAB-03989) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (human CD44)) Monoclonal Antibody, Unconjugated (TMAB-03989) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded Human Esophagus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with human CD44 Monoclonal Antibody, Unconjugated (TMAB-03989) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
7. Paraformaldehyde-fixed, paraffin embedded Human Cervical Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with human CD44 Monoclonal Antibody, Unconjugated (TMAB-03989) at 1:200 overnight at 4°C, followed by conjugation to the HRP and DAB staining.
8. Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with human CD44 Monoclonal Antibody, Unconjugated (TMAB-03989) at 1:200 overnight at 4°C, followed by conjugation to the HRP and DAB staining.
9. Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with human CD44 Monoclonal Antibody, Unconjugated (TMAB-03989) at 1:200 overnight at 4°C, followed by conjugation to the HRP and DAB staining.
10. Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with human CD44 Monoclonal Antibody, Unconjugated (TMAB-03989) at 1:200 overnight at 4°C, followed by conjugation to the HRP and DAB staining.
11. Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD44 Monoclonal Antibody, Unconjugated (TMAB-03989) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Mouse IgG antibody (Green, BF488), DAPI (blue) was used to stain the cell nuclei.
12. Paraformaldehyde-fixed, paraffin embedded Human Esophagus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD44 Monoclonal Antibody, Unconjugated (TMAB-03989) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Mouse IgG antibody (Green, BF488), DAPI (blue) was used to stain the cell nuclei.

IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:100-500; IF: 1:100-500; FCM: 1μg/Test

Monoclonal

KLH conjugated synthetic peptide: human CD44

Human

CD44

CD44 antigen

Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events.