Anti-CD44 Antibody (4R653) is a Mouse antibody targeting CD44. Anti-CD44 Antibody (4R653) can be used in FCM,ICC/IF,IF,IHC-Fr,IHC-P,WB.

IgG2a

4R653

Human

1. Paraformaldehyde-fixed, paraffin embedded (Human pancreatic cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CD44) Monoclonal Antibody, Unconjugated (TMAB-00368) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
2. Blank control: Hela.
Primary Antibody (green line): Mouse Anti-CD44 antibody (TMAB-00368)
Dilution: 1 μg/Test;
Secondary Antibody (white blue line): Goat anti-mouse IgG-AF488
Dilution: 0.5 μg/Test.
Isotype control (orange line): Normal Mouse IgG
Protocol
The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
3. 25 μg total protein per Lane of various lysates probed with CD44 monoclonal antibody, unconjugated (TMAB-00368) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min.
4. Paraformaldehyde-fixed, paraffin embedded Human Cervical Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD44 Monoclonal Antibody, Unconjugated (TMAB-00368) at 1:150 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD44 Monoclonal Antibody, Unconjugated (TMAB-00368) at 1:150 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD44 Monoclonal Antibody, Unconjugated (TMAB-00368) at 1:150 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
7. Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD44 Monoclonal Antibody, Unconjugated (TMAB-00368) at 1:150 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
8. Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD44 Monoclonal Antibody, Unconjugated (TMAB-00368) at 1:150 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
9. Paraformaldehyde-fixed, paraffin embedded Human Esophagus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD44 Monoclonal Antibody, Unconjugated (TMAB-00368) at 1:150 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
10. Paraformaldehyde-fixed, paraffin embedded Human Esophagus Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD44 Monoclonal Antibody, Unconjugated (TMAB-00368) at 1:150 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

WB: 1:200-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:100-500; IF: 1:100-500; FCM: 1ug/Test

Monoclonal

Recombinant Protein: human CD44

Human

CD44

CD44 antigen

Tumor biomarkers,CD markers,Adhesion,CD markers ELISA kits,T Lymphocytic Lineage,Dendritic Cell Lineage,Neutrophil Lineage,Surface Molecules

Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events.