Anti-CD41 Polyclonal Antibody is a Rabbit antibody targeting CD41. Anti-CD41 Polyclonal Antibody can be used in FCM.

IgG

Human,Rat (predicted:Mouse,Cow,Horse,Rabbit)

1. Blank control (blue line): HL60 cells (fixed with 70% methanol (Overnight at 4°C)).
Primary Antibody (green line): Rabbit Anti-Integrin alpha 2b antibody (TMAB-00367), Dilution: 1 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE, Dilution: 1 μg/test.
2. Blank control: U937. Primary Antibody (green line): Rabbit Anti-CD41/Integrin alpha 2b antibody (TMAB-00367)
Dilution: 1 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-AF647
Dilution: 1 μg/test.
Protocol
The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.

FCM: 1μg /test

Polyclonal

KLH conjugated synthetic peptide: human Integrin alpha 2b heavy chain

Human

ITGA2B

Integrin alpha-IIb

Integrins,Platelets,Platelets,Alpha,Murine Lineage Negative,Thrombocytic Lineage

Isoform 1 and isoform 2 were identified in platelets and megakaryocytes, but not in reticulocytes or in Jurkat and U937 white blood cell line. Isoform 3 is expressed by leukemia, prostate adenocarcinoma and melanoma cells but not by platelets or normal prostate or breast epithelial cells.