Anti-CD41 Polyclonal Antibody is a Rabbit antibody targeting CD41. Anti-CD41 Polyclonal Antibody can be used in FCM.

IgG

Human,Rat (predicted:Mouse,Cow,Horse,Rabbit)

1. Blank control (blue line): HL60 cells (fixed with 70% methanol (Overnight at 4°C)).
Primary Antibody (green line): Rabbit Anti-Integrin alpha 2b antibody (TMAB-00367), Dilution: 1 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE, Dilution: 1 μg/test.
2. Blank control: U937. Primary Antibody (green line): Rabbit Anti-CD41/Integrin alpha 2b antibody (TMAB-00367)
Dilution: 1 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-AF647
Dilution: 1 μg/test.
Protocol
The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.

FCM

FCM: 1μg /test

Polyclonal

KLH conjugated synthetic peptide: human Integrin alpha 2b heavy chain

Human

Integrins,Platelets,Platelets,Alpha,Murine Lineage Negative,Thrombocytic Lineage