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Anti-CD40L Polyclonal Antibody
Catalog No. TMAB-03984 Copy Product Info
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Anti-CD40L Polyclonal Antibody is a Rabbit antibody targeting CD40L. Anti-CD40L Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
Anti-CD40L Polyclonal Antibody
Copy Product Info🥰Excellent
Catalog No. TMAB-03984
Anti-CD40L Polyclonal Antibody is a Rabbit antibody targeting CD40L. Anti-CD40L Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
Anti-CD40L Polyclonal Antibody
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 50 μL | $221 | 7-10 days | 7-10 days | |
| 100 μL | $373 | 7-10 days | 7-10 days | |
| 200 μL | $527 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
Stability & Storage
| Description | Anti-CD40L Polyclonal Antibody is a Rabbit antibody targeting CD40L. Anti-CD40L Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Human,Rat |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (rat spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CD40L) Polyclonal Antibody, Unconjugated (TMAB-03984) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded (rat spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CD40L) Polyclonal Antibody, Unconjugated (TMAB-03984) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded (human liver cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CD40L) Polyclonal Antibody, Unconjugated (TMAB-03984) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining. |
| Application | |
| Recommended Dose | IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Secreted and Cell membrane. |
| Tissue Specificity | Specifically expressed on activated CD4+ T-lymphocytes. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | CD40 ligand (CD40L) is a 33 kDa type II membrane glycoprotein expressed mainly on the cell surface of activated T lymphocytes, but also exists as a soluble form extracellularly. CD40L is the ligand for CD40, a member of the TNF superfamily, which is expressed on the cell surface of B cells, macrophages/monocytes, dendritic cells, vascular endothelial cells, and epithelial cells. CD40L plays an important role in B cell proliferation, antibody class switching, modulation of apoptosis in the germinal center through interaction with B cells expressing CD40, and activation of CD4+ T cells. Mutation within the CD40L gene is linked to hyper IgM syndrome, an X linked immunodeficiency disease that is characterized by elevated level of serum IgM and decreased level of other isotypes. |
| Immunogen | KLH conjugated synthetic peptide: human CD40L |
| Antigen Species | Human |
| Gene Name | CD40LG |
| Gene ID | |
| Protein Name | CD40 ligand |
| Uniprot ID | |
| Function | Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching.
Release of soluble CD40L from platelets is partially regulated by GP IIb/IIIa, actin polymerization, and an matrix metalloproteinases (MMP) inhibitor-sensitive pathway. |
| Molecular Weight | Theoretical: 29 kDa. Actual: 32-39 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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