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Anti-CD40 Polyclonal Antibody
😃Good
Catalog No. TMAB-00365
Anti-CD40 Polyclonal Antibody is a Rabbit antibody targeting CD40. Anti-CD40 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-CD40 Polyclonal Antibody
Anti-CD40 Polyclonal Antibody
😃Good
Catalog No. TMAB-00365
Anti-CD40 Polyclonal Antibody is a Rabbit antibody targeting CD40. Anti-CD40 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $222 | 7-10 days | |
| 100 μL | $372 | 7-10 days | |
| 200 μL | $528 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Antibody Type: Rabbit Polyclonal Application: WB,IHC-P,IHC-Fr,IF Reactivity: Human,Mouse,Rat,Rabbit (predicted:Chicken,Dog,Pig,Cow) |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat,Rabbit (predicted:Chicken,Dog,Pig,Cow) |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (human tonsil tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CD40) Polyclonal Antibody, Unconjugated (TMAB-00365) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 2. Sample: Lane 1: Spleen (Mouse) Lysate at 40 μg Lane 2: Lung (Mouse) Lysate at 40 μg Lane 3: LympHnode (Rat) Lysate at 40 μg Primary: Anti-CD40/TNFRSF5 (TMAB-00365) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 44-50 kDa Observed band size: 50 kDa 3. Sample: Hela (Human) Cell Lysate at 30 μg Primary: Anti-CD40 (TMAB-00365) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 30 kDa Observed band size: 52 kDa 4. Sample: Jurkat (Human) Cell Lysate at 30 μg Raw264.7 (Mouse) Cell Lysate at 30 μg Primary: Anti-CD40 (TMAB-00365) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 30 kDa Observed band size: 52 kDa |
| Application | WB,IHC-P,IHC-Fr,IF |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Isoform I: Cell membrane; Single-pass type I membrane protein. Isoform II: Secreted. |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | Predicted to enable antigen binding activity; protein domain specific binding activity; and ubiquitin protein ligase binding activity. Involved in B cell mediated immunity; CD40 signaling pathway; and cellular calcium ion homeostasis. Acts upstream of or within several processes, including defense response to other organism; positive regulation of B cell activation; and positive regulation of interleukin-12 production. Located in external side of plasma membrane and intracellular membrane-bounded organelle. Part of CD40 receptor complex. Is expressed in several structures, including alimentary system; brain; hemolymphoid system gland; liver and biliary system; and reproductive system. Human ortholog(s) of this gene implicated in several diseases, including Kawasaki disease; autoimmune disease (multiple); end stage renal disease; hyperimmunoglobulin syndrome (multiple); and non-Hodgkin lymphoma (multiple). Orthologous to human CD40 (CD40 molecule). [provided by Alliance of Genome Resources, Apr 2022] |
| Immunogen | KLH conjugated synthetic peptide: human CD40/TNFRSF5. |
| Antigen Species | Human |
| Gene ID | 958 |
| Uniprot ID | |
| Biology Area | Innate and adaptive immunity,CD,B Lymphocytic Lineage,Dendritic Cell Lineage |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
Sci Citations
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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