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Anti-CD40 Antibody (8Z859)

😃Good
Catalog No. TMAY-01158
Alias TRAP, Tnfrsf5, T-BAM, p50, IMD3, Igm, HIGM1, gp39, CD40 molecule, TNF receptor superfamily member 5, Bp50, AI326936

Anti-CD40 Antibody (8Z859) is a Mouse antibody targeting CD40. Anti-CD40 Antibody (8Z859) can be used in FCM,ICC/IF.

Anti-CD40 Antibody (8Z859)

Anti-CD40 Antibody (8Z859)

😃Good
Catalog No. TMAY-01158Alias TRAP, Tnfrsf5, T-BAM, p50, IMD3, Igm, HIGM1, gp39, CD40 molecule, TNF receptor superfamily member 5, Bp50, AI326936
Anti-CD40 Antibody (8Z859) is a Mouse antibody targeting CD40. Anti-CD40 Antibody (8Z859) can be used in FCM,ICC/IF.
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100 μL$198 7-10 days
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Product Introduction

Bioactivity
Description
Anti-CD40 Antibody (8Z859) is a Mouse antibody targeting CD40. Anti-CD40 Antibody (8Z859) can be used in FCM,ICC/IF.
AliasTRAP, Tnfrsf5, T-BAM, p50, IMD3, Igm, HIGM1, gp39, CD40 molecule, TNF receptor superfamily member 5, Bp50, AI326936
Ig Type
Monoclonal Mouse IgG1
Clone
8Z859
Reactivity
Human
Verified Activity
1. Immunofluorescence staining of Human CD40 in Hep G2 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Mouse anti-Human CD40 monoclonal antibody (1:60) at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (left panel, captured by laser confocal scanning microscope; right panel, captured by fluorescence microscope), countstained with DAPI (blue). Positive staining was localized to cytoplasm and cytomembrane.

2. Flow cytometric analysis of human CD40 expression on human whole blood lymphocytes. Human whole blood lymphocytes were stained with purified anti-Human CD40, then a FITC-conjugated second step antibody. The histogram were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes.
Application
FCM,ICC/IF
Recommended Dose
ICC-IF: 1:20-1:100; FCM: 1:25-1:100
Antibody Type
Monoclonal
Host SpeciesMouse
ConstructionThis antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD40 / TNFRSF5 (rh CD40 / TNFRSF5; TMPY-01181; NP_001241.1; Met1-Arg193). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
PurificationProtein A
AppearanceLiquid
Formulation0.2 μm filtered solution in PBS
Research BackgroundCD40, also known as TNFRSF5, is a member of the TNF receptor superfamily which are single transmembrane-spanning glycoproteins. CD40 protein plays an essential role in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. CD40 protein is expressed in B cells, dendritic cells, macrophages, endothelial cells, and several tumor cell lines. Defects in CD40 result in hyper-IgM immunodeficiency type 3 (HIGM3). In addition, CD40/CD40L interaction is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis.Cancer ImmunotherapyCo-stimulatory Immune Checkpoint TargetsImmune CheckpointImmune Checkpoint Detection: AntibodiesImmune Checkpoint Detection: ELISA AntibodiesImmune Checkpoint Detection: FCM AntibodiesImmune Checkpoint Detection: ICC AntibodiesImmune Checkpoint Detection: IP AntibodiesImmune Checkpoint Detection: WB AntibodiesImmune Checkpoint ProteinsImmune Checkpoint TargetsImmunotherapyTargeted Therapy
Related Conjugates and Formulations
Conjucates
Unconjugated
Others Formats
APC/FITC/PE
Antibody Types Available
4
Antigen Details
Immunogen
Recombinant Human CD40 / TNFRSF5 protein (TMPY-01181)
Antigen Species
Human
Biology Area
Cancer Drug Targets
Chemical Properties
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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