Anti-CD4 Polyclonal Antibody is a Rabbit antibody targeting CD4. Anti-CD4 Polyclonal Antibody can be used in FCM,IF,IHC-Fr,IHC-P,WB.

IgG

Human,Mouse,Pig (predicted:Rat,Dog,Cow,Sheep,GuineaPig,MACFA)

1. Image was kindly submitted by Dr.David M Burmeister from US Army Institute of Surgical Research. Pig lympHnodes stained with Rabbit Anti-CD4 Polyclonal Antibody and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature).
2. Blank control (blue line): HL60 (fixed with 2% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature).
Primary Antibody (green line): Rabbit Anti-CD4 antibody (TMAB-00362),Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC,dilution: 1μg /test.
3. Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CD4) Polyclonal Antibody, Unconjugated (TMAB-00362) at 1:4000 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded (human tonsil); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CD4) Polyclonal Antibody, Unconjugated (TMAB-00362) at 1:4000 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
5. Sample:
Lane 1: U937 (Human) Cell Lysate at 30 μg
Lane 2: HL-60 (Human) Cell Lysate at 30 μg
Lane 3: Jurkat (Human) Cell Lysate at 30 μg
Primary: Anti-CD4 (TMAB-00362) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55 kDa
Observed band size: 55 kDa
6. Sample:
Spleen (Mouse) Lysate at 40 μg
Primary: Anti-CD4 (TMAB-00362) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48 kDa
Observed band size: 55 kDa
7. Sample:
LympHnode (Mouse) Lysate at 40 μg
Primary: Anti-CD4 (TMAB-00362) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48 kDa
Observed band size: 55 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1μg/Test

Polyclonal

KLH conjugated synthetic peptide: human CD4

Human

CD4

T-cell surface glycoprotein CD4

Regulatory T Cells,CD,HIV-related,T Lymphocytic Lineage,Dendritic Cell Lineage,Monocytic Lineage

Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts.