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Anti-CD4 Polyclonal Antibody
😃Good
Catalog No. TMAB-00362
Alias Ly-4, L3T4, CD4 molecule
Anti-CD4 Polyclonal Antibody is a Rabbit antibody targeting CD4. Anti-CD4 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.
Anti-CD4 Polyclonal Antibody
Anti-CD4 Polyclonal Antibody
😃Good
Catalog No. TMAB-00362Alias Ly-4, L3T4, CD4 molecule
Anti-CD4 Polyclonal Antibody is a Rabbit antibody targeting CD4. Anti-CD4 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $222 | 7-10 days | |
| 100 μL | $373 | 7-10 days | |
| 200 μL | $527 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-CD4 Polyclonal Antibody is a Rabbit antibody targeting CD4. Anti-CD4 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM. |
| Alias | Ly-4, L3T4, CD4 molecule |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Pig (predicted:Rat,Dog,Cow,Sheep,GuineaPig,MACFA) |
| Verified Activity | 1. Image was kindly submitted by Dr.David M Burmeister from US Army Institute of Surgical Research. Pig lympHnodes stained with Rabbit Anti-CD4 Polyclonal Antibody and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature). Primary Antibody (green line): Rabbit Anti-CD4 antibody (TMAB-00362), Dilution: 1 μg/10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, Dilution: 1 μg/test. 3. Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CD4) Polyclonal Antibody, Unconjugated (TMAB-00362) at 1:4000 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded (human tonsil); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CD4) Polyclonal Antibody, Unconjugated (TMAB-00362) at 1:4000 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 5. Sample: Lane 1: U937 (Human) Cell Lysate at 30 μg Lane 2: HL-60 (Human) Cell Lysate at 30 μg Lane 3: Jurkat (Human) Cell Lysate at 30 μg Primary: Anti-CD4 (TMAB-00362) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 55 kDa Observed band size: 55 kDa 6. Sample: Spleen (Mouse) Lysate at 40 μg Primary: Anti-CD4 (TMAB-00362) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 48 kDa Observed band size: 55 kDa 7. Sample: LympHnode (Mouse) Lysate at 40 μg Primary: Anti-CD4 (TMAB-00362) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 48 kDa Observed band size: 55 kDa |
| Application | WB,IHC-P,IHC-Fr,IF,FCM |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1μg/Test |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cell membrane; Single-pass type I membrane protein. Note=Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum. |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | This gene encodes a membrane glycoprotein of T lymphocytes that interacts with major histocompatibility complex class II antigenes and is also a receptor for the human immunodeficiency virus. This gene is expressed not only in T lymphocytes, but also in B cells, macrophages, and granulocytes. It is also expressed in specific regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been identified in this gene. [provided by RefSeq, Aug 2010]. |
| Immunogen | KLH conjugated synthetic peptide: human CD4 |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | Regulatory T Cells,CD,HIV-related,T Lymphocytic Lineage,Dendritic Cell Lineage,Monocytic Lineage |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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