Shopping Cart
  • Remove All
  • TargetMol
    Your shopping cart is currently empty

Anti-CD4 Antibody (2J310)

😃Good
Catalog No. TMAY-03133
Alias Ly-4, L3T4, CD4 molecule

Anti-CD4 Antibody (2J310) is a Rabbit antibody targeting CD4. Anti-CD4 Antibody (2J310) can be used in FCM.

Anti-CD4 Antibody (2J310)

Anti-CD4 Antibody (2J310)

😃Good
Catalog No. TMAY-03133Alias Ly-4, L3T4, CD4 molecule
Anti-CD4 Antibody (2J310) is a Rabbit antibody targeting CD4. Anti-CD4 Antibody (2J310) can be used in FCM.
Pack SizePriceAvailabilityQuantity
100 μL$270 7-10 days
Bulk & Custom
Add to Cart
Questions
View More
Contact us for more batch information
All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

Product Introduction

Bioactivity
Description
Anti-CD4 Antibody (2J310) is a Rabbit antibody targeting CD4. Anti-CD4 Antibody (2J310) can be used in FCM.
AliasLy-4, L3T4, CD4 molecule
Ig Type
Monoclonal Rabbit IgG
Clone
2J310
Reactivity
Mouse
Verified Activity
Flow cytometric analysis of Mouse CD4 expression on BABL/c splenocytes. Cells were stained with purified anti-Mouse CD4, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
Application
FCM
Recommended Dose
FCM: 1:25-1:100
Antibody Type
Monoclonal
Host SpeciesRabbit
ConstructionThis antibody was obtained from a rabbit immunized with purified, recombinant Mouse CD4 (rM CD4; TMPY-01226; NP_038516.1; Met1-Thr394).
PurificationProtein A
AppearanceLiquid
Formulation0.2 μm filtered solution in PBS
Research BackgroundT-cell surface glycoprotein CD4, is a single-pass type I membrane protein. CD4 contains three Ig-like C2-type (immunoglobulin-like) domains and one Ig-like V-type (immunoglobulin-like) domain. CD4 is a glycoprotein expressed on the surface of T helper cells, regulatory T cells, monocytes, macrophages, and dendritic cells. The CD4 surface determinant, previously associated as a phenotypic marker for helper/inducer subsets of T lymphocytes, has now been critically identified as the binding/entry protein for human immunodeficiency viruses (HIV). The human CD4 molecule is readily detectable on monocytes, T lymphocytes, and brain tissues. All human tissue sources of CD4 bind radiolabeled gp120 to the same relative degree; however, the murine homologous protein, L3T4, does not bind the HIV envelope protein. CD4 is a co-receptor that assists the T cell receptor (TCR) to activate its T cell following an interaction with an antigen-presenting cell. Using its portion that resides inside the T cell, CD4 amplifies the signal generated by the TCR. CD4 interacts directly with MHC class II molecules on the surface of the antigen-presenting cell via its extracellular domain. The CD4 molecule is currently the object of intense interest and investigation both because of its role in normal T-cell function, and because of its role in HIV infection. CD4 is a primary receptor used by HIV-1 to gain entry into host T cells. HIV infection leads to a progressive reduction of the number of T cells possessing CD4 receptors.Viral protein U (VpU) of HIV-1 plays an important role in downregulation of the main HIV-1 receptor CD4 from the surface of infected cells. Physical binding of VpU to newly synthesized CD4 in the endoplasmic reticulum is an early step in a pathway leading to proteasomal degradation of CD4. Amino acids in both helices found in the cytoplasmic region of VpU in membrane-mimicking detergent micelles experience chemical shift perturbations upon binding to CD4, whereas amino acids between the two helices and at the C-terminus of VpU show no or only small changes, respectively. Paramagnetic spin labels were attached at three sequence positions of a CD4 peptide comprising the transmembrane and cytosolic domains of the receptor. VpU binds to a membrane-proximal region in the cytoplasmic domain of CD4.
Related Conjugates and Formulations
Conjucates
Unconjugated
Others Formats
APC/FITC/PE
Antibody Types Available
4
Antigen Details
Immunogen
Recombinant Mouse CD4 Protein (TMPY-01226)
Antigen Species
Mouse
Chemical Properties
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
TransportShipping with blue ice.

Sci Citations

Calculator

  • Molarity Calculator
  • Dilution Calculator
  • Reconstitution Calculator
  • Molecular Weight Calculator

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
Related Tags: buy Anti-CD4 Antibody (2J310) | purchase Anti-CD4 Antibody (2J310) | Anti-CD4 Antibody (2J310) cost | order Anti-CD4 Antibody (2J310)