Anti-CD3D Antibody (5T24) (Synonyms: T3D, IMD19, CD3-δ, CD3-DELTA, CD3d molecule, δ (CD3-TCR complex), CD3d...)

Name: Anti-CD3D Antibody (5T24)
Brand: TargetMol
SKU: TMAY-01414
Rating: 4.5 (1 reviews)

Catalog No. TMAY-01414 Copy Product Info

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Anti-CD3D Antibody (5T24) is a Mouse antibody targeting CD3D. Anti-CD3D Antibody (5T24) can be used in IP,WB.

Anti-CD3D Antibody (5T24)

Copy Product Info

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Catalog No. TMAY-01414

Synonyms T3D, IMD19, CD3-δ, CD3-DELTA, CD3d molecule, δ (CD3-TCR complex), CD3d molecule, delta (CD3-TCR complex), CD3 δ, CD3 delta

Anti-CD3D Antibody (5T24) is a Mouse antibody targeting CD3D. Anti-CD3D Antibody (5T24) can be used in IP,WB.

Anti-CD3D Antibody
(5T24)

Pack Size	Price	USA Stock	Global Stock	Quantity
100 μL	$199	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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Product Introduction

Bioactivity

Description	Anti-CD3D Antibody (5T24) is a Mouse antibody targeting CD3D. Anti-CD3D Antibody (5T24) can be used in IP,WB.
Synonyms	T3D, IMD19, CD3-δ, CD3-DELTA, CD3d molecule, δ (CD3-TCR complex), CD3d molecule, delta (CD3-TCR complex), CD3 δ, CD3 delta
Ig Type	Mouse IgG1
Clone	5T24
Reactivity	Human
Specificity	Human CD3 delta/CD3D
Verified Activity	1. CD3D was immunoprecipitated using: -Lane A:0.5 mg HuT78 Whole Cell Lysate. -2 µL anti-CD3D mouse monoclonal antibody and 60 μg of Immunomagnetic beads Protein G. -Primary antibody: -Anti-CD3D mouse monoclonal antibody, at 1:100 dilution. -Secondary antibody: -Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilution. -Developed using the odyssey technique. -Performed under reducing conditions. -Predicted band size: 20 kDa. -Observed band size: 20 kDa. 2. Anti-CD3D mouse monoclonal antibody at 1:500 dilution. -Lane A: Jurkat Whole Cell Lysate. -Lane B: Molt-4 Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:19 kDa. -Observed band size:24 kDa
Application	IP WB
Recommended Dose	WB: 1:500-1:2000; IP: 4-6 μL/mg of lysate
Antibody Type	Monoclonal
Host Species	Mouse
Construction	This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD3d / CD3 delta (rh CD3d / CD3 delta; TMPY-01196; NP_000723.1; Met1-Ala105). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Purification	Protein A
Appearance	Liquid
Formulation	0.2 μm filtered solution in PBS
Research Background	T-cell surface glycoprotein CD3 delta chain, also known as CD3D, is a single-pass type I membrane protein. CD3D, together with CD3-gamma, CD3-epsilon and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. The majority of T cell receptor (TCR) complexes in mice and humans consist of a heterodimer of polymorphic TCR alpha and beta chains along with invariant CD3 gamma, delta, epsilon, and zeta chains. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. This complex is critical for T-cell development and function, and represents one of the most complex transmembrane receptors. The T cell receptor-CD3 complex is unique in having ten cytoplasmic immunoreceptor tyrosine-based activation motifs (ITAMs). CD3D contains 1 ITAM domain and has been shown to interact with CD8A. In the mouse, knockout of CD3delta allows some degree of T lymphocyte differentiation since mature CD4 and CD8 as well as TCRgammadelta T lymphocytes are observed in the periphery. In contrast, deleterious mutation of the CD3 delta encoding gene in the human leads to a severe combined immunodeficiency characterised by the complete absence of mature T cell subpopulations including TCRalpha/beta and TCR gamma/delta. Defects in CD3D cause severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T-/B+/NK+ SCID) which is a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. In humans the absence of CD3 delta results in a complete arrest in thymocyte development at the stage of double negative to double positive transition and the development of gamma delta T-cell receptor-positive T cells is also impaired.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy

Related Conjugates and Formulations

Conjucates

Unconjugated

Antigen Details

Immunogen	Recombinant Protein: Human CD3d / CD3 delta Protein (TMPY-01196)
Antigen Species	Human

Chemical Properties

Stability & Storage

Stability & Storage	Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
Transport	Shipping with blue ice.