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Anti-CD361 Polyclonal Antibody

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Catalog No. TMAB-03965

Anti-CD361 Polyclonal Antibody is a Rabbit antibody targeting CD361. Anti-CD361 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-CD361 Polyclonal Antibody

Anti-CD361 Polyclonal Antibody

Copy Product Info
😃Good
Catalog No. TMAB-03965
Anti-CD361 Polyclonal Antibody is a Rabbit antibody targeting CD361. Anti-CD361 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack SizePriceUSA WarehouseGlobal WarehouseQuantity
50 μL$2227-10 days7-10 days
100 μL$3747-10 days7-10 days
200 μL$5277-10 days7-10 days
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction

Bioactivity
Description
Anti-CD361 Polyclonal Antibody is a Rabbit antibody targeting CD361. Anti-CD361 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
Reactivity
Human,Mouse
Verified Activity
1. Sample: Cerebrum (Mouse) Lysate at 40 μg
Primary: Anti-CD361 (TMAB-03965) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 46 kD
Observed band size: 66 kD
2. Tissue/cell: mosue embryo tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-CD361 Polyclonal Antibody, Unconjugated (TMAB-03965) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
3. Tissue/cell: Human lung cancer tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-CD361 Polyclonal Antibody, Unconjugated (TMAB-03965) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
4. Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-CD361 Polyclonal Antibody, Unconjugated (TMAB-03965) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
Application
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationMembrane.
Tissue SpecificityBone marrow, peripheral blood mononuclear cells, fibroblasts and Epstein-Barr virus-transformed lymphoblastoid cell lines.
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundEVI2B is a 448 amino acid protein which functions in the differentiation of melanocytes and keratinocytes. Lying within an intron of the Neurofibromin gene, the gene encoding EVI2B is transcribed from the telomere toward the centromere, which is opposite the transcription direction of the Neurofibromin gene. EVI2B is a single-pass transmembrane protein containing an extracellular domain with 4 glycosylation sites, a N-terminal signal peptide, a cytoplasmic hydrophilic domain and a hydrophobic transmembrane domain. Due to evidence suggesting that gene encoding the mouse homolog lies within a viral integration site that has been identitified in retrovirus-induced myeloid tumors, the gene encoding EVI2B may function as an oncogene in these tumor types. With expression in peripheral blood mononuclear cells, fibroblasts, bone marrow and EBV-transformed lymphoblastoid cell lines, EVI2B is implicated in leukemogenesis.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human CD361
Antigen Species
Human
Gene Name
EVI2B
Gene ID
Protein Name
Protein EVI2B
Uniprot ID
Chemical Properties
Molecular WeightTheoretical: 46 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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