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Anti-CD34 Antibody (1K692)

😃Good
Catalog No. TMAY-00259
Alias CD34 molecule, AU040960

Anti-CD34 Antibody (1K692) is a Mouse antibody targeting CD34. Anti-CD34 Antibody (1K692) can be used in WB,IP.

Anti-CD34 Antibody (1K692)

Anti-CD34 Antibody (1K692)

😃Good
Catalog No. TMAY-00259Alias CD34 molecule, AU040960
Anti-CD34 Antibody (1K692) is a Mouse antibody targeting CD34. Anti-CD34 Antibody (1K692) can be used in WB,IP.
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100 μL$198 7-10 days
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Product Introduction

Bioactivity
Description
Anti-CD34 Antibody (1K692) is a Mouse antibody targeting CD34. Anti-CD34 Antibody (1K692) can be used in WB,IP.
AliasCD34 molecule, AU040960
Ig Type
Monoclonal Mouse IgG1
Clone
1K692
Reactivity
Human
Verified Activity
1. Anti-CD34 mouse monoclonal antibody at 1:500 dilution.

-Lane A: TF-1 Whole Cell lysate.

-Lysates/proteins at 30 μg per lane.

-Secondary

-Goat Anti-Mouse IgG (H+L)/HRP at 1/10000 dilution.

-Developed using the ECL technique.

-Performed under reducing conditions.

-Predicted band size:41 kDa.

2. pCMV3-CD34-TT-WPRE was immunoprecipitated using:

-Lane A:0.5 mg TF-1 Whole Cell Lysate.

-4 µL anti-pCMV3-CD34-TT-WPRE mouse monoclonal antibody and 60 μg of Immunomagnetic beads Protein A/G.

-Primary antibody:

-Anti-pCMV3-CD34-TT-WPRE mouse monoclonal antibody, at 1:100 dilution.

-Secondary antibody:

-Rabbit Anti-Mouse IgG F(ab)2/HRP at 1/10000 dilution.

-Developed using the ECL technique.

-Performed under reducing conditions.

-Predicted band size: 41 kDa.

-Observed band size:95 kDa
Application
WB,IP
Recommended Dose
WB: 1:500-1:2000; IP: 1-5 μL/mg of lysate
Antibody Type
Monoclonal
Host SpeciesMouse
ConstructionThis antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with full length human CD34 expression plasmid. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
PurificationProtein A
AppearanceLiquid
Formulation0.2 μm filtered solution in PBS
Research BackgroundCluster of Differentiation 34 (CD34) is a member of a family of single-pass transmembrane sialomucin proteins, and may function as a cell-cell adhesion factor. CD34 protein is selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues. It has been widely used as a stem and progenitor cell marker, and clinical CD34+ stem cell transplantation (CD34+SCT) has been performed for tumor purging. CD34 monoclonal antibodies are widely used to identify and isolate hemopoietic progenitors and to classify acute and chronic leukemias.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Full length human CD34 expression plasmid
Antigen Species
Human
Biology Area
Cardiac Stem Cell Markers, Hemangioblast Markers
Chemical Properties
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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