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Anti-CD34 Antibody (1K692) is a Mouse antibody targeting CD34. Anti-CD34 Antibody (1K692) can be used in WB,IP.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
100 μL | $198 | 7-10 days |
Description | Anti-CD34 Antibody (1K692) is a Mouse antibody targeting CD34. Anti-CD34 Antibody (1K692) can be used in WB,IP. |
Alias | CD34 molecule, AU040960 |
Ig Type | Monoclonal Mouse IgG1 |
Clone | 1K692 |
Reactivity | Human |
Verified Activity | 1. Anti-CD34 mouse monoclonal antibody at 1:500 dilution. -Lane A: TF-1 Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Mouse IgG (H+L)/HRP at 1/10000 dilution. -Developed using the ECL technique. -Performed under reducing conditions. -Predicted band size:41 kDa. 2. pCMV3-CD34-TT-WPRE was immunoprecipitated using: -Lane A:0.5 mg TF-1 Whole Cell Lysate. -4 µL anti-pCMV3-CD34-TT-WPRE mouse monoclonal antibody and 60 μg of Immunomagnetic beads Protein A/G. -Primary antibody: -Anti-pCMV3-CD34-TT-WPRE mouse monoclonal antibody, at 1:100 dilution. -Secondary antibody: -Rabbit Anti-Mouse IgG F(ab)2/HRP at 1/10000 dilution. -Developed using the ECL technique. -Performed under reducing conditions. -Predicted band size: 41 kDa. -Observed band size:95 kDa |
Application | WB,IP |
Recommended Dose | WB: 1:500-1:2000; IP: 1-5 μL/mg of lysate |
Antibody Type | Monoclonal |
Host Species | Mouse |
Construction | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with full length human CD34 expression plasmid. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
Purification | Protein A |
Appearance | Liquid |
Formulation | 0.2 μm filtered solution in PBS |
Research Background | Cluster of Differentiation 34 (CD34) is a member of a family of single-pass transmembrane sialomucin proteins, and may function as a cell-cell adhesion factor. CD34 protein is selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues. It has been widely used as a stem and progenitor cell marker, and clinical CD34+ stem cell transplantation (CD34+SCT) has been performed for tumor purging. CD34 monoclonal antibodies are widely used to identify and isolate hemopoietic progenitors and to classify acute and chronic leukemias. |
Conjucates | Unconjugated |
Immunogen | Full length human CD34 expression plasmid |
Antigen Species | Human |
Biology Area | Cardiac Stem Cell Markers, Hemangioblast Markers |
Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
Transport | Shipping with blue ice. |
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