Anti-CD33 Polyclonal Antibody is a Rabbit antibody targeting CD33. Anti-CD33 Polyclonal Antibody can be used in WB,FCM.

IgG

Human

1. 20 ng rhCD33-His protein per lane probed with CD33 polyclonal antibody respectively, unconjugated (TMAB-03956) at 1:1000 dilution and 4°C overnight incubation. Followed by corresponding conjugated secondary antibody incubation at r. T. for 60 min.
2. The THP-1 (H) cells incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.). Primary Antibody (green): Rabbit Anti-CD33 antibody (TMAB-03956): 1 μg/10^6 cells; Secondary Antibody (white blue): Goat anti-Rabbit IgG-FITC: 1 μg/test. Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.
3. 25 μg total protein per lane of various lysates (see on figure) probed with CD33 polyclonal antibody, unconjugated (TMAB-03956) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.

WB: 1:500-2000; FCM: 1μg/Test

Polyclonal

Recombinant Protein: human CD33 protein

Human

CD33

Myeloid cell surface antigen CD33

Putative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. Induces apoptosis in acute myeloid leukemia (in vitro).