Anti-CD32 Polyclonal Antibody 2 is a Rabbit antibody targeting CD32. Anti-CD32 Polyclonal Antibody 2 can be used in WB,FCM.

IgG

Human

1. Sample: K562 Cell Lysate at 30 μg
Primary: Anti-CD32 (TMAB-03947) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 31 kD
Observed band size: 33 kD
2. Blank control: U937 (blue). Primary Antibody: Rabbit Anti-CD32 antibody (TMAB-03947), Dilution: 0.2 μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions); Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), Dilution: 1: 200 in 1 X PBS containing 0.5% BSA.
Protocol
The cells were fixed with 2% paraformaldehyde (10 min). Primary antibody (TMAB-03947, 0.2 μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA+1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.
3. Sample:
Jurkat (Human) Cell Lysate at 30 μg
Primary: Anti-CD32 (TMAB-03947) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 31 kD
Observed band size: 31 kD

WB: 1:500-2000; FCM: 0.2μg/Test

Polyclonal

KLH conjugated synthetic peptide: human Fc gamma RII a

Human

FCGR2A

Low affinity immunoglobulin gamma Fc region receptor II-a

Binds to the Fc region of immunoglobulins gamma. Low affinity receptor. By binding to IgG it initiates cellular responses against pathogens and soluble antigens. Promotes phagocytosis of opsonized antigens.