Anti-CD32/FcRII-b Antibody (6K455) is a Mouse antibody targeting CD32/FcRII-b. Anti-CD32/FcRII-b Antibody (6K455) can be used in WB,IHC-P,IHC-Fr,IF.

IgG

6K455

Human

1. Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD32/FcRII-b Monoclonal Antibody, Unconjugated (TMAB-03953) at 1: 200 overnight at 4°C, followed by conjugation to the AffiniPure Goat Anti-Mouse IgG H&L-HRP and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD32/FcRII-b Monoclonal Antibody, Unconjugated (TMAB-03953) at 1: 200 overnight at 4°C, followed by conjugation to the AffiniPure Goat Anti-Mouse IgG H&L-HRP and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Human Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD32/FcRII-b Monoclonal Antibody, Unconjugated (TMAB-03953) at 1: 200 overnight at 4°C, followed by conjugation to the AffiniPure Goat Anti-Mouse IgG H&L-HRP and DAB staining.
4. 25 μg total protein per lane of various lysates (see on figure) probed with CD32/FcRII-b monoclonal antibody, unconjugated (TMAB-03953) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Monoclonal

Recombinant Protein: Human Fc gamma RIIB/C (CD32b/c)

Human

FCGR2B

Low affinity immunoglobulin gamma Fc region receptor II-b

Binds to the Fc region of immunoglobulins gamma. Low affinity receptor. By binding to IgG it initiates cellular responses against pathogens and soluble antigens. Promotes phagocytosis of opsonized antigens.