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Anti-CD32 Antibody (4Z96)

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Catalog No. TMAB-03949 Copy Product Info
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Anti-CD32 Antibody (4Z96) is a Rabbit antibody targeting CD32. Anti-CD32 Antibody (4Z96) can be used in WB,IHC-P,IHC-Fr,IF.

Anti-CD32 Antibody
(4Z96)

Pack SizePriceUSA StockGlobal StockQuantity
25 µL$1507-10 days7-10 days
50 µL$2627-10 days7-10 days
100 µL$4727-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-CD32 Antibody (4Z96) is a Rabbit antibody targeting CD32. Anti-CD32 Antibody (4Z96) can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
Clone
4Z96
Reactivity
Human (predicted:Mouse,Rat)
Verified Activity
1. Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP)
Lysate: 1: Human placenta, 2: Human liver
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 34 kDa
Observed MW: 34 kDa
2. Tissue: Human placenta
Section type: Formalin-fixed & Paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for
3. Tissue: Human liver
Section type: Formalin-fixed & Paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 1 hour at room temperature
Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for TMAB-03949
Application
Recommended Dose
WB: 1:500-1000; IHC-P: 1:100-500; IHC-Fr: 1:200-400; IF: 1:100-500
Antibody Type
Monoclonal
Host SpeciesRabbit
Subcellular LocalizationCell membrane; Single-pass type I membrane protein.
Tissue SpecificityIs the most broadly distributed Fc-gamma-receptor. Expressed in monocyte, neutrophils, macrophages, basophils, eosinophils, Langerhans cells, B-cells, platelets cells and placenta (endothelial cells). Not detected in natural killer cells.
ConstructionRecombinant Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundThe protein encoded by this gene is a low affinity receptor for the Fc region of immunoglobulin gamma complexes. The encoded protein is involved in the phagocytosis of immune complexes and in the regulation of antibody production by B-cells. Variations in this gene may increase susceptibilty to systemic lupus erythematosus (SLE). Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010]
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human CD32
Antigen Species
Human
Gene Name
FCGR2B
Gene ID
Protein Name
Low affinity immunoglobulin gamma Fc region receptor II-b
Uniprot ID
Function
Binds to the Fc region of immunoglobulins gamma. Low affinity receptor. By binding to IgG it initiates cellular responses against pathogens and soluble antigens. Promotes phagocytosis of opsonized antigens.
Chemical Properties
Molecular WeightTheoretical: 31 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
Related Tags: Anti-CD32 Antibody (4Z96) molecular weight