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Anti-CD31 Polyclonal Antibody

😃Good
Catalog No. TMAB-00355
Alias PECAM-1, PECA1, GPIIA', FLJ58394, FLJ34100, EndoCAM, CD31

Anti-CD31 Polyclonal Antibody is a Rabbit antibody targeting CD31. Anti-CD31 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.

Anti-CD31 Polyclonal Antibody

Anti-CD31 Polyclonal Antibody

😃Good
Catalog No. TMAB-00355Alias PECAM-1, PECA1, GPIIA', FLJ58394, FLJ34100, EndoCAM, CD31
Anti-CD31 Polyclonal Antibody is a Rabbit antibody targeting CD31. Anti-CD31 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
Pack SizePriceAvailabilityQuantity
50 μL$2217-10 days
100 μL$3737-10 days
200 μL$5297-10 days
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Product Introduction

Bioactivity
Description
Anti-CD31 Polyclonal Antibody is a Rabbit antibody targeting CD31. Anti-CD31 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
AliasPECAM-1, PECA1, GPIIA', FLJ58394, FLJ34100, EndoCAM, CD31
Ig Type
IgG
Reactivity
Human (predicted:Sheep,Rabbit,Cow,Pig,Dog)
Verified Activity
1. Paraformaldehyde-fixed, paraffin embedded (human tonsil); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CD31) Polyclonal Antibody, Unconjugated (TMAB-00355) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CD31) Polyclonal Antibody, Unconjugated (TMAB-00355) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded (human tonsil); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (CD31) Polyclonal Antibody, Unconjugated (TMAB-00355) at 1:200 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody for 90 minutes, and DAPI for nucleus staining.
4. Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD31 Polyclonal Antibody, Unconjugated (TMAB-00355) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD31 Polyclonal Antibody, Unconjugated (TMAB-00355) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD31 Polyclonal Antibody, Unconjugated (TMAB-00355) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
7. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD31 Polyclonal Antibody, Unconjugated (TMAB-00355) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
Application
IHC-P,IHC-Fr,IF
Recommended Dose
IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationIsoform Long: Membrane; Single-pass type I membrane protein. Cell junction. Note=Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells.Isoform Delta15: Cell junction. Note=Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundThe protein encoded by this gene is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. The encoded protein is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation. [provided by RefSeq, May 2010]
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human CD31
Antigen Species
Human
Gene ID
Uniprot ID
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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