Anti-CD31 Antibody (2K688) is a Mouse antibody targeting CD31. Anti-CD31 Antibody (2K688) can be used in WB,IHC-P,IHC-Fr,IF,FCM.

IgG

2K688

Human

1. Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD31 Monoclonal Antibody, Unconjugated (TMAB-00354) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green), DAPI (blue) was used to stain the cell nucleus.
2. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD31 Monoclonal Antibody, Unconjugated (TMAB-00354) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green), DAPI (blue) was used to stain the cell nucleus.
3. Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD31 Monoclonal Antibody, Unconjugated (TMAB-00354) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green), DAPI (blue) was used to stain the cell nucleus.
4. Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD31 Monoclonal Antibody, Unconjugated (TMAB-00354) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green), DAPI (blue) was used to stain the cell nucleus.
5. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD31 Monoclonal Antibody, Unconjugated (TMAB-00354) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green), DAPI (blue) was used to stain the cell nucleus.
6. Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD31 Monoclonal Antibody, Unconjugated (TMAB-00354) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Red), DAPI (blue) was used to stain the cell nucleus.
7. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD31 Monoclonal Antibody, Unconjugated (TMAB-00354) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Red), DAPI (blue) was used to stain the cell nucleus.
8. Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD31 Monoclonal Antibody, Unconjugated (TMAB-00354) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Red), DAPI (blue) was used to stain the cell nucleus.
9. 25 μg total protein per Lane of various lysates probed with CD31 monoclonal antibody, unconjugated (TMAB-00354) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min.
10. The THP-1 (H) cells were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at room temperature). Primary Antibody (green): Mouse Anti-CD31 antibody (TMAB-00354): 0.5 μg/10^6 cells; Secondary Antibody (white blue): Goat anti-Mouse IgG-FITC for 15 min; The section was incubated with CD31 Monoclonal Antibody, Unconjugated (ascites of TMAB-00354) at 1:1500 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
12. Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD31 Monoclonal Antibody, Unconjugated (ascites of TMAB-00354) at 1:1500 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
13. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD31 Monoclonal Antibody, Unconjugated (ascites of TMAB-00354) at 1:1500 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
14. Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD31 Monoclonal Antibody, Unconjugated (ascites of TMAB-00354) at 1:1500 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
15. Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD31 Monoclonal Antibody, Unconjugated (ascites of TMAB-00354) at 1:1500 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
16. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD31 Monoclonal Antibody, Unconjugated (ascites of TMAB-00354) at 1:1500 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
17. Paraformaldehyde-fixed, paraffin embedded Human Duodenum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD31 Monoclonal Antibody, Unconjugated (ascites of TMAB-00354) at 1:1500 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
18. 4% Paraformaldehyde-fixed THP-1 (H) cell; Antibody incubation with (CD31) monoclonal Antibody, unconjugated (TMAB-00354) 1:200, 90 min at 37°C; followed by conjugated Goat Anti-Mouse IgG antibody (green) at 37°C for 90 min, DAPI (blue) was used to stain the cell nucleus. PBS instead of the primary antibody was used as the blank control.

WB,IHC-P,IHC-Fr,IF,FCM

WB: 1:100-1000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 0.5ug/Test

Monoclonal

Recombinant Protein: human CD31 28-601/727aa (C-6x His-Tag)

Human