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Anti-CD3 epsilon Antibody (7O149)
Catalog No. TMAB-03935 Copy Product Info
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Anti-CD3 epsilon Antibody (7O149) is a Rabbit antibody targeting CD3 epsilon. Anti-CD3 epsilon Antibody (7O149) can be used in IF, IHC-Fr, IHC-P, WB.
Anti-CD3 epsilon Antibody (7O149)
Copy Product Info🥰Excellent
Catalog No. TMAB-03935
Anti-CD3 epsilon Antibody (7O149) is a Rabbit antibody targeting CD3 epsilon. Anti-CD3 epsilon Antibody (7O149) can be used in IF, IHC-Fr, IHC-P, WB.
Anti-CD3 epsilon Antibody
(7O149)
(7O149)
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 50 μL | $264 | 7-10 days | 7-10 days | |
| 100 μL | $473 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction
Bioactivity
Antigen Details
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| Description | Anti-CD3 epsilon Antibody (7O149) is a Rabbit antibody targeting CD3 epsilon. Anti-CD3 epsilon Antibody (7O149) can be used in IF, IHC-Fr, IHC-P, WB. |
| Ig Type | IgG |
| Clone | 7O149 |
| Reactivity | Human |
| Verified Activity | 1. Sample: Lane 1: Molt-4 (Human) Cell Lysate at 30 ug Lane 2: Jurkat (Human) Cell Lysate at 30 ug Primary: Anti-CD3 epsilon (TMAB-03935) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 23 kD Observed band size: 23 kD 2. Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CD3 epsilon) Monoclonal Antibody, Unconjugated (TMAB-03935) at 1:50 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded (Human tonsil); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CD3 epsilon) Monoclonal Antibody, Unconjugated (TMAB-03935) at 1:50 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded (Human gastric cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CD3 epsilon) Monoclonal Antibody, Unconjugated (TMAB-03935) at 1:50 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
| Application | |
| Recommended Dose | IF=1:50-200; IHC-Fr=1:50-200; IHC-P=1:50-200; WB=1:500-2000 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Subcellular Localization | Membrane; Single-pass type I membrane protein. |
| Construction | Recombinant Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | CD3e molecule, epsilon is also known as CD3E, is a T-cell surface single-pass type I membrane glycoprotein. CD3E contains 1 Ig-like (immunoglobulin-like) domain and 1 ITAM domain. CD3E, together with CD3-gamma, CD3-delta and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. CD3E plays an essential role in T-cell development, and defects in CD3E gene cause severe immunodeficiency. CD3E gene has also been linked to a susceptibility to type I diabetes in women. CD3E has been shown to interact with TOP2B, CD3EAP and NCK2. |
| Immunogen | Recombinant Protein: human CD3 epsilon |
| Antigen Species | Human |
| Gene Name | CD3E |
| Gene ID | |
| Protein Name | T-cell surface glycoprotein CD3 epsilon chain |
| Uniprot ID | |
| Function | The CD3 complex mediates signal transduction. |
| Molecular Weight | Theoretical: 20 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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