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Anti-CD28 Polyclonal Antibody 4
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Catalog No. TMAB-03932
Anti-CD28 Polyclonal Antibody 4 is a Rabbit antibody targeting CD28. Anti-CD28 Polyclonal Antibody 4 can be used in IHC-P,WB,IHC-Fr,IF.
Anti-CD28 Polyclonal Antibody 4
Anti-CD28 Polyclonal Antibody 4
Copy Product Info😃Good
Catalog No. TMAB-03932
Anti-CD28 Polyclonal Antibody 4 is a Rabbit antibody targeting CD28. Anti-CD28 Polyclonal Antibody 4 can be used in IHC-P,WB,IHC-Fr,IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $220 | 7-10 days | 7-10 days | |
| 100 μL | $373 | 7-10 days | 7-10 days | |
| 200 μL | $529 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
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| Description | Anti-CD28 Polyclonal Antibody 4 is a Rabbit antibody targeting CD28. Anti-CD28 Polyclonal Antibody 4 can be used in IHC-P,WB,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Human (predicted:Dog,Horse,Rabbit) |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (human tonsil); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (CD28 Rabbit pAb) Polyclonal Antibody, Unconjugated (TMAB-03932) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. 2. Sample: Lane 1: Recombinant human CD28 protein, C-hFc (HEK193) Primary: Anti-CD28 (TMAB-03932) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 25 kDa Observed band size: 60 kDa |
| Application | |
| Recommended Dose | IHC-P: 1:100-500; WB: 1:500-2000; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Membrane; Single-pass type I membrane protein. |
| Tissue Specificity | Expressed in T-cells and plasma cells, but not in less mature B-cells. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | The protein encoded by this gene is essential for T-cell proliferation and survival, cytokine production, and T-helper type-2 development. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jul 2011] |
| Immunogen | Recombinant Protein: human CD28 protein |
| Antigen Species | Human |
| Gene Name | CD28 |
| Gene ID | |
| Protein Name | T-cell-specific surface glycoprotein CD28 |
| Uniprot ID | |
| Function | Possibly involved in T-cell activation. |
| Molecular Weight | Theoretical: 25 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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