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Anti-CD25 Antibody (3Y634)
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Catalog No. TMAB-03926
Anti-CD25 Antibody (3Y634) is a Rabbit antibody targeting CD25. Anti-CD25 Antibody (3Y634) can be used in FCM,WB.
Anti-CD25 Antibody (3Y634)
Anti-CD25 Antibody (3Y634)
Copy Product Info😃Good
Catalog No. TMAB-03926
Anti-CD25 Antibody (3Y634) is a Rabbit antibody targeting CD25. Anti-CD25 Antibody (3Y634) can be used in FCM,WB.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 25 μL | $148 | 7-10 days | 7-10 days | |
| 50 μL | $263 | 7-10 days | 7-10 days | |
| 100 μL | $471 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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| Description | Anti-CD25 Antibody (3Y634) is a Rabbit antibody targeting CD25. Anti-CD25 Antibody (3Y634) can be used in FCM,WB. |
| Ig Type | IgG |
| Clone | 3Y634 |
| Reactivity | Human |
| Verified Activity | 1. The Jurkat (treated with 20 ng/ml PMA for 24 hours) (H) cells were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-CD25 antibody (TMAB-03926): 1 μg/10^6 cells; Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed. 2. 25 μg total protein per lane of various lysates (see on figure) probed with CD25 monoclonal antibody, unconjugated (TMAB-03926) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min. |
| Application | |
| Recommended Dose | FCM: 1μg/Test; WB: 1:500-2000 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Subcellular Localization | Membrane; Single-pass type I membrane protein. |
| Construction | Recombinant Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency. Patients with severe Coronavirus Disease 2019 (COVID-19), the disease caused by the novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), have significantly elevated levels of IL2R in their plasma. Similarly, serum IL-2R levels are found to be elevated in patients with different types of carcinomas. Certain IL2RA and IL2RB gene polymorphisms have been associated with lung cancer risk. [provided by RefSeq, Jul 2020] |
| Gene Name | IL2RA |
| Gene ID | |
| Protein Name | Interleukin-2 receptor subunit alpha |
| Function | Receptor for interleukin-2. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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