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Anti-CD25 Antibody (3Y634)

Catalog No. TMAB-03926 Copy Product Info
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Anti-CD25 Antibody (3Y634) is a Rabbit antibody targeting CD25. Anti-CD25 Antibody (3Y634) can be used in FCM,WB.

Anti-CD25 Antibody (3Y634)

Copy Product Info
🥰Excellent
Catalog No. TMAB-03926

Anti-CD25 Antibody (3Y634) is a Rabbit antibody targeting CD25. Anti-CD25 Antibody (3Y634) can be used in FCM,WB.

Anti-CD25 Antibody
(3Y634)
Pack SizePriceUSA StockGlobal StockQuantity
25 μL$1487-10 days7-10 days
50 μL$2637-10 days7-10 days
100 μL$4717-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-CD25 Antibody (3Y634) is a Rabbit antibody targeting CD25. Anti-CD25 Antibody (3Y634) can be used in FCM,WB.
Ig Type
IgG
Clone
3Y634
Reactivity
Human
Verified Activity
1. The Jurkat (treated with 20 ng/ml PMA for 24 hours) (H) cells were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-CD25 antibody (TMAB-03926): 1 μg/10^6 cells; Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.
2. 25 μg total protein per lane of various lysates (see on figure) probed with CD25 monoclonal antibody, unconjugated (TMAB-03926) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
Application
FCM
WB
Recommended Dose
FCM: 1μg/Test; WB: 1:500-2000
Antibody Type
Monoclonal
Host SpeciesRabbit
Subcellular LocalizationMembrane; Single-pass type I membrane protein.
ConstructionRecombinant Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundThe interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency. Patients with severe Coronavirus Disease 2019 (COVID-19), the disease caused by the novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), have significantly elevated levels of IL2R in their plasma. Similarly, serum IL-2R levels are found to be elevated in patients with different types of carcinomas. Certain IL2RA and IL2RB gene polymorphisms have been associated with lung cancer risk. [provided by RefSeq, Jul 2020]
Antigen Details
Gene Name
IL2RA
Gene ID
3559
Protein Name
Interleukin-2 receptor subunit alpha
Function
Receptor for interleukin-2.
Chemical Properties
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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