Anti-CD23 Antibody (3M534) is a Mouse antibody targeting CD23. Anti-CD23 Antibody (3M534) can be used in IHC-P,IHC-Fr,IF,FCM.

IgG2b

3M534

Human

1. The Daudi (H) cells were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.). Primary Antibody (green): Mouse Anti-CD23 antibody (TMAB-03917): 1 μg/10^6 cells; Secondary Antibody (white blue): Goat anti-Mouse IgG-FITC: 1 μg/test. Blank control (black): PBS. Acquisition of 20,000 events was performed.
2. Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD23 Monoclonal Antibody, Unconjugated (TMAB-03917) at 1: 200 overnight at 4°C, followed by conjugation to the AffiniPure Goat Anti-Mouse IgG H&L-HRP and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Human Lymph; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD23 Monoclonal Antibody, Unconjugated (TMAB-03917) at 1: 200 overnight at 4°C, followed by conjugation to the AffiniPure Goat Anti-Mouse IgG H&L-HRP and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD23 Monoclonal Antibody, Unconjugated (TMAB-03917) at 1: 200 overnight at 4°C, followed by conjugation to the AffiniPure Goat Anti-Mouse IgG H&L-HRP and DAB staining.
5. (Negative control) Paraformaldehyde-fixed, paraffin embedded Human Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD23 Monoclonal Antibody, Unconjugated (TMAB-03917) at 1: 200 overnight at 4°C, followed by conjugation to the AffiniPure Goat Anti-Mouse IgG H&L-HRP and DAB staining.

IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1μg/Test

Monoclonal

Recombinant Protein: human CD23

Human

FCER2

Low affinity immunoglobulin epsilon Fc receptor

Low-affinity receptor for immunoglobulin E (IgE) and CR2/CD21. Has essential roles in the regulation of IgE production and in the differentiation of B-cells (it is a B-cell-specific antigen).